PMID: 3769917Oct 1, 1986Paper

Porcine submaxillary mucin contains alpha 2----3- and alpha 2----6-linked N-acetyl- and N-glycolylneuraminic acid

European Journal of Biochemistry
A V SavageD H Van den Eijnden

Abstract

Four acidic trisaccharides have been obtained by alkaline borohydride reductive cleavage (beta-elimination) of a fraction of porcine submaxillary mucin precipitating at 60-75% ethanol. Their structures have been investigated using the techniques of methylation analysis involving gas-liquid chromatography/mass spectrometry along with high-resolution 1H-NMR analysis. Two of the four oligosaccharides, B1-B4, contain N-acetylneuraminic acid (NeuAc) while two contain N-glycolylneuraminic acid (NeuGc). The following structures are proposed for the acidic trisaccharide fraction: (B1) NeuAc alpha(2----3)Gal beta(1----3)GalNAcol, (B2) NeuGc alpha(2----3)Gal beta(1----3)GalNAcol, (B3) Gal beta(1----3)[NeuAc alpha(2----6)]GalNAcol and (B4) Gal beta(1----3)[NeuGc alpha(2----6)]GalNAcol, (GalNAcol = reduced N-acetylgalactosamine. These oligosaccharides were present in a molar ratio of 69:22:4:5. Although oligosaccharide B4 has previously been found in porcine submaxillary mucin B1, B2 and B3 have not. Furthermore, oligosaccharide B2 is a novel structure.

References

Jan 15, 1979·Molecular and Cellular Biochemistry·A HerpJ Moschera
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Jun 1, 1977·Carbohydrate Research·H Rauvala, J Kärkkäinen
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Feb 1, 1981·Hoppe-Seyler's Zeitschrift für physiologische Chemie·D H van den EijndenW E Schiphorst
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Jun 26, 1980·Biochimica Et Biophysica Acta·H van HalbeekP Jolles

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Citations

Apr 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T UrashimaT Nakamura
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·A P Neeleman, D H van de Eijnden
May 23, 2015·Molecular & Cellular Proteomics : MCP·Katalin F MedzihradszkyRobert J Chalkley
Sep 20, 1996·The Journal of Biological Chemistry·G H TaiI A Nieduszynski
Jan 14, 1999·The American Journal of Physiology·B J van KlinkenJ Dekker

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