Porcine SWC1 is CD52--final determination by the use of a retroviral cDNA expression library.

Veterinary Immunology and Immunopathology
Judith LeitnerArmin Saalmüller

Abstract

During the last decades for several species--e.g. swine--many mAb to leukocyte-specific molecules have been developed and clusters of differentiation corresponding to human CD could be established. However, for a significant amount of the raised mAb the corresponding antigens were not characterized on the molecular level and therefore preliminary clusters--in swine so-called Swine workshop clusters (SWC)--were established. These clusters contain antigens with currently no obvious orthologs to human leukocyte differentiation antigens. In this study, we describe the generation of a eukaryotic cDNA expression library from in vitro activated porcine peripheral blood mononuclear cells. Screening of this library with an antibody recognizing SWC1 enabled isolation and sequencing of cDNAs coding for the porcine SWC1 molecule. A BLAST search of the obtained sequence revealed that SWC1 is the orthologous molecule of human CD52. Therefore, our study provides the basis for comparative studies on the role of CD52 in different mammalian species. In addition, the established cDNA library can be used for investigation of additional SWC-defined molecules.

References

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Citations

Feb 25, 2014·Developmental and Comparative Immunology·Corinna SedlakWilhelm Gerner
Jul 19, 2014·Developmental and Comparative Immunology·Corinna SedlakWilhelm Gerner
Nov 20, 2014·Wiener klinische Wochenschrift·I Popow
Mar 19, 2013·Veterinary Research·Katharina ReutnerWilhelm Gerner
Aug 4, 2012·Developmental and Comparative Immunology·Katharina ReutnerWilhelm Gerner
Feb 28, 2016·Developmental and Comparative Immunology·Irene M Rodríguez-GómezWilhelm Gerner
Oct 31, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·I PopowP Steinberger
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Mar 31, 2021·Developmental and Comparative Immunology·Jemma V MilburnWilhelm Gerner

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Methods Mentioned

BETA
flow
flow cytometry
electrophoresis
PCR
immunoprecipitation

Software Mentioned

FACSDiva
PI Predictor
big
BLAST
CELLQUEST

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