Porcine transmissible gastroenteritis virus inhibits NF-κB activity via nonstructural protein 3 to evade host immune system

Virology Journal
Yanan WangLi Wang

Abstract

Transmissible gastroenteritis virus (TGEV), a member of the family Coronaviridae, causes lethal watery diarrhea in piglets. Previous studies have revealed that the coronaviruses develop various strategies to evade the host innate immunity through the inhibition of nuclear factor kappa B (NF-κB) signaling pathway. However, the ability of TGEV to inhibit the host innate immune response by modulating the NF-κB signaling pathway is not clear. In this study, a dual luciferase reporter assay was used to confirm the inhibition of NF-κB by TGEV infection and to identify the major viral proteins involved in the inhibition of NF-κB signaling. Real-time quantitative PCR was used to quantify the mRNA expression of inflammatory factors. The deubiquitination of Nsp3 domains and its effect on IκBα and p65 were analyzed by western blotting. The ubiquitination level of IκBα was analyzed by immunoprecipitation. In ST and IPEC-J2 cells, TGEV exhibited a dose-dependent inhibition of NF-κB activity. Individual TGEV protein screening revealed the high potential of non-structural protein 3 (Nsp3) to inhibit NF-κB signaling, and leading to the downregulation of the NF-κB-induced cytokine production. We demonstrated that the inhibitory effect of Nsp3 w...Continue Reading

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Citations

Jun 25, 2020·Microbiology and Immunology·Fumihiro KatoMakoto Takeda
Oct 5, 2020·Microbial Pathogenesis·Zhiwei LiShuqi Xiao

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Datasets Mentioned

BETA
KU729220

Methods Mentioned

BETA
ubiquitination
nuclear translocation
transfection
PCR
protein assay
electrophoresis
co-immunoprecipitation
nuclear
deubiquitination

Software Mentioned

GraphPad Prism
SMART

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