Pore formation in a binary giant vesicle induced by cone-shaped lipids.

Biophysical Journal
Yuka SakumaMasayuki Imai

Abstract

We have investigated shape deformations of binary giant unilamellar vesicles (GUVs) composed of cone- and cylinder-shaped lipids. By coupling the spontaneous curvature of lipids with the phase separation, we demonstrated pore opening and closing in GUVs. When the temperature was set below the chain melting transition temperature of the cylinder-shaped lipid, the GUVs burst and then formed a single large pore, where the cone shape lipids form a cap at the edge of the bilayer to stabilize the pore. The pore closed when we increased the temperature above the transition temperature. The pore showed three types of shapes depending on the cone-shaped lipid concentration: simple circular, rolled-rim, and wrinkled-rim pores. These pore shape changes indicate that the distribution of the cone- and cylinder-shaped lipids is asymmetric between the inner and outer leaflets in the bilayer. We have proposed a theoretical model for a two-component membrane with an edge of bilayer where lipids can transfer between two leaflets. Using this model, we have reproduced numerically the observed shape deformations at the rim of pore.

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Citations

Apr 17, 2014·The Journal of Chemical Physics·Chloe M FunkhouserK Thornton
Mar 5, 2015·Life·Yuka Sakuma, Masayuki Imai
Jul 25, 2014·Nature Communications·Yong-Sang RyuSin-Doo Lee
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Jun 29, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Takashi TaniguchiMasayuki Imai
Jan 15, 2016·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Hiroki HimenoMasahiro Takagi
May 21, 2011·Physical Chemistry Chemical Physics : PCCP·Radek ŠachlLennart B-Å Johansson
Mar 15, 2015·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Ashkan DehghanAn-Chang Shi
Dec 21, 2011·Physical Review Letters·Yuka Sakuma, Masayuki Imai
Oct 21, 2019·Biophysical Chemistry·Tayana Mazin TsuboneRosangela Itri
Oct 4, 2012·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Yukitaka IshimotoRyoichi Yamamoto
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Jun 22, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Shengwen ZhangPaul A Beales
Mar 21, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jingfei ChenJingcheng Hao
Dec 12, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Norifumi L Yamada
May 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Maïwenn BeaugrandIsabelle Marcotte
Mar 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Raymond MalabedKaori Sakurai
Jun 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Sana AhmedTsutomu Hamada
Jun 28, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Svetlana S EfimovaOlga S Ostroumova

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