Pores from mitochondrial outer membranes of yeast and a porin-deficient yeast mutant: a comparison.

Journal of Bioenergetics and Biomembranes
R BenzM Dihanich

Abstract

Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused by the formation of ion-permeable channels in the membranes. Yeast porin has a single-channel conductance of 4.2 nS in 1 M KCl. In the open state it behaves as a general diffusion pore with an effective diameter of 1.7 nm and possesses properties similar to other mitochondrial porins. Surprisingly, the membrane conductance also increased in the presence of detergent extracts of the mitochondrial outer membrane of the mutant. Single-channel recordings of lipid bilayer membranes in the presence of small concentration of the mutant membranes suggested that this membrane also contained a pore. The reconstituted pores had a single-channel conductance of 2.0 nS in 1 M KCl and the characteristics of general diffusion pores with an estimated effective diameter of 1.2 nm. This means that the pores present in the mitochondrial outer membranes of the yeast mutant have a much smaller effective diameter than "normal" mitochondr...Continue Reading

References

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Citations

Aug 1, 1989·Journal of Bioenergetics and Biomembranes·H TedeschiC A Mannella
Aug 10, 1992·FEBS Letters·I I Pottosin
Aug 1, 1989·Journal of Bioenergetics and Biomembranes·E Blachly-DysonM Forte
Aug 1, 1993·FEMS Microbiology Reviews·G J SulterM Veenhuis
Jan 11, 2018·Scientific Reports·Natascha A WolffFrank Thévenod
Apr 24, 2018·Frontiers in Chemistry·Andrea MagrìVito De Pinto
Apr 12, 1995·Biochimica Et Biophysica Acta·I SzabóM Zoratti

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