PMID: 9171398Jun 1, 1997Paper

Porin polypeptide contributes to surface charge of gonococci

Journal of Bacteriology
J SwansonD Kao

Abstract

Each strain of Neisseria gonorrhoeae elaborates a single porin polypeptide, with the porins expressed by different strains comprising two general classes, Por1A and Por1B. In the outer membrane, each porin molecule folds into 16 membrane-spanning beta-strands joined by top- and bottom-loop domains. Por1A and Por1B have similar membrane-spanning regions, but the eight surface-exposed top loops (I to VIII) differ in length and sequence. To determine whether porins, and especially their top loop domains, contribute to bacterial cell surface charge, strain MS11 gonococci that were identical except for expressing a recombinant Por1A, Por1B, or mosaic Por1A-1B polypeptide were compared by whole-cell electrophoresis. These porin variants displayed different electrophoretic mobilities that correlated with the net numbers of charged amino acids within surface-exposed loops of their respective porin polypeptides. The susceptibilities of porin variants to polyanionic sulfated polymers correlated roughly with gonococcal surface charge; those porin variants with diminished surface negativity showed increased sensitivity to the polyanionic sulfated compounds. These observations indicate that porin polypeptides in situ contribute to the surfa...Continue Reading

References

Oct 1, 1976·Journal of General Microbiology·J E HeckelsM E Ward
Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Sep 20, 1992·Journal of Molecular Biology·M S Weiss, G E Schulz
May 29, 1992·Cell·J P Zhang, R S Stephens
Sep 1, 1991·Infection and Immunity·P van der LeyJ T Poolman
Sep 1, 1991·Molecular Microbiology·D JeanteurF Pattus
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S Meri, M K Pangburn
May 11, 1990·Journal of General Microbiology·M E Bayer, J L Sloyer
Jan 1, 1991·Methods in Enzymology·A D CardinR L Jackson
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·N H CarbonettiP F Sparling
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E C GotschlichM Koomey
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N H Carbonetti, P F Sparling
Jul 1, 1974·Journal of Bacteriology·K H Johnston, E C Gotschlich
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J D YoungZ A Cohn
Jun 1, 1994·FEMS Microbiology Letters·M J GillC A Ison
Aug 1, 1995·The Journal of Experimental Medicine·T ChenJ Swanson
Jul 22, 1994·Journal of Molecular Biology·A KarshikoffT Schirmer
Jan 1, 1997·Infection and Immunity·K S Rostand, J D Esko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.