Porosity variation in hydroxyapatite and osteoblast morphology: a scanning electron microscopy study

Journal of Microscopy
B AnnazL Di Silvio

Abstract

The biocompatibility of hydroxyapatite has been demonstrated by previous studies, with enhancement of osteointegration through the use of porous hydroxyapatite (pHA). Emphasis has been focused on the use of coralline hydroxyapatite or the introduction of macroporosity into synthetic hydroxyapatite. The current study investigates the role of macro- and microporosities in synthetic phase-pure porous hydroxyapatite on the morphological aspects of human osteoblast-like cells using scanning electron microscopy. Cells were seeded on four different types of porous hydroxyapatite (HA1, HA2, HA3 and HA4) and examined following 1, 2, 14 and 30 days of incubation in vitro. The results indicated that the cells had an affinity to micropores through filopodia extensions, at initial stage of attachment. Cellular proliferation and colonization was evident on all materials with cells forming cellular bridges across the macropores at day 14 with cellular canopy formation covering entire macropores observed by day 30. This study demonstrates that while the introduction of microporosity has no evident effect on cellular morphology at later time points, it seems to play a role in initial cellular anchorage and attachment.

Citations

Sep 25, 2007·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Makoto MotomiyaAkio Minami
May 6, 2005·Journal of Materials Science. Materials in Medicine·K A HingT Buckland
Apr 18, 2006·Journal of Materials Science. Materials in Medicine·Masanori OkamotoHiroshi Hosoi
Feb 6, 2007·Journal of Materials Science. Materials in Medicine·Hockin H K XuCarl G Simon
Oct 8, 2008·Journal of Materials Science. Materials in Medicine·D BoydI M Brook
Mar 6, 2012·Journal of Materials Science. Materials in Medicine·Jennifer H ShepherdSerena M Best
Jan 25, 2013·Journal of Materials Science. Materials in Medicine·Shin-Hee JunYoung-Hag Koh
Jul 17, 2012·Photomedicine and Laser Surgery·Nai-Chia TengHaw-Ming Huang
Sep 10, 2011·Journal of Tissue Engineering·David BrindleyIvan Wall
May 8, 2014·BioMed Research International·Joshua O EniwumideMasatsugu Shimomura
May 15, 2013·Acta Biomaterialia·S J PolakA J Wagoner Johnson
Dec 1, 2009·Acta Biomaterialia·Pamela HabibovicClemens A van Blitterswijk
Oct 27, 2015·Frontiers in Bioengineering and Biotechnology·Maria Isabella Gariboldi, Serena M Best
May 31, 2007·Clinical Implant Dentistry and Related Research·Johan MalmströmPeter Thomsen
Jun 27, 2012·Journal of Biomedical Materials Research. Part a·Kate CameronBrendon Noble
Mar 9, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Charlie R CampionKarin Hing
Mar 16, 2012·Journal of Biomedical Materials Research. Part a·Melanie J CoathupGordon W Blunn
May 9, 2012·Journal of Biomedical Materials Research. Part a·Hye-Sun YuHae-Won Kim
Aug 22, 2016·Acta Biomaterialia·Laurence E RustomAmy J Wagoner Johnson
Jul 14, 2011·Journal of Biomaterials Applications·Shekhar NathBikramjit Basu
Nov 25, 2017·Bio-medical Materials and Engineering·Fahimeh Sadat TabatabaeiSaeed Tatari
Nov 13, 2004·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Karin A Hing
Apr 22, 2006·Journal of Biomedical Materials Research. Part a·Myriam RouahiKarine Anselme
Jul 17, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·J L XuR Kumar
Aug 11, 2007·Journal of Biomedical Materials Research. Part a·Angela M H NgB H I Ruszymah
Dec 29, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Samir K GhoshDebabrata Basu
Apr 6, 2006·Journal of Biomedical Materials Research. Part a·Alexandra E PorterWilliam Bonfield
Mar 19, 2020·Journal of Biomaterials Science. Polymer Edition·Sandhya SinghNarayan Chand Mishra
Jul 11, 2020·Journal of Biomaterials Science. Polymer Edition·Sandhya SinghNarayan Chand Mishra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.