Porous hybrid structures based on P(DLLA-co-TMC) and collagen for tissue engineering of small-diameter blood vessels

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Laura ButtafocoJan Feijen

Abstract

Poly (D,L-lactide)-7co-(1,3-trimethylene carbonate) [P(DLLA-co-TMC)] (83 mol % DLLA) was used to produce matrices suitable for tissue engineering of small-diameter blood vessels. The copolymer was processed into tubular structures with a porosity of approximately 98% by melt spinning and fiber winding, thus obviating the need of organic solvents that may compromise subsequent cell culture. Unexpectedly, incubation in culture medium at 37 degrees C resulted in disconnection of the contact points between the polymer fibers. To improve the structural stability of these P(DLLA-co-TMC) scaffolds, a collagen microsponge was formed inside the pores of the synthetic matrix by dip coating and freeze drying. Hybrid structures with a porosity of 97% and an average pore size of 102 mum were obtained. Structural stability was preserved during incubation in culture medium at 37 degrees C. Smooth-muscle cells (SMCs) were seeded in these hybrid scaffolds and cultured under pulsatile flow conditions in a bioreactor (120 beats/min, 80-120 mmHg). After 7 days of culture in a dynamic environment viable SMCs were homogeneously distributed throughout the constructs, which were five times stronger and stiffer than noncultured scaffolds. Values for yi...Continue Reading

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Nov 18, 2005·Biomaterials·Laura ButtafocoJan Feijen

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Citations

Jan 13, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hajar SeyednejadWim E Hennink
Apr 5, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Li LiuDeyue Yan
Mar 29, 2014·Journal of Biomedical Materials Research. Part a·Po-Yang ChenLynn L H Huang
Aug 15, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D I BraghirolliP Pranke
Mar 5, 2011·The International Journal of Artificial Organs·Yan SongDirk W Grijpma

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