Abstract
A proof of concept for the label-free detection of bacteriophage MS2, a model indicator of microbiological contamination, is validated in this work as a porous silicon (pSi) membrane-based electrochemical biosensor. PSi membranes were used to afford nanochannel architectures. The sensing mechanism was based on the nanochannel blockage caused by MS2 binding to immobilized capture antibodies. This blockage was quantified by measuring the oxidation current of the electroactive species reaching the electrode surface, by means of differential pulse voltammetry (DPV). The immunosensor showed a limit of detection of 6 pfu/mL in buffer, allowing the detection of MS2 to levels commonly found in real-world applications, and proved to be unaffected by matrix effects when analyzing MS2 in reservoir water. This platform enables the straightforward, direct and sensitive detection of a broad range of target analytes and constitutes a promising approach towards the development of portable electronic point of sample analysis devices.
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Jun 22, 2019·Chemical Communications : Chem Comm·Maria AlbaNicolas H Voelcker
Sep 7, 2019·Materials·Valerii Myndrul, Igor Iatsunskyi
Jan 31, 2020·Analytical and Bioanalytical Chemistry·Jonathan ZoreaHadar Ben-Yoav
Jan 10, 2021·Water Research·Kang MaoZhugen Yang
Jan 26, 2018·ACS Applied Materials & Interfaces·Nekane RetaNicolas H Voelcker
Apr 7, 2018·ACS Applied Materials & Interfaces·Rui LiuJianlin Li
Nov 19, 2019·Analytical Chemistry·Wenyue ZhuYingchun Fu
May 30, 2019·ACS Sensors·Nekane RetaNicolas H Voelcker