Porous silicon membrane-modified electrodes for label-free voltammetric detection of MS2 bacteriophage

Biosensors & Bioelectronics
Nekane RetaNicolas H Voelcker

Abstract

A proof of concept for the label-free detection of bacteriophage MS2, a model indicator of microbiological contamination, is validated in this work as a porous silicon (pSi) membrane-based electrochemical biosensor. PSi membranes were used to afford nanochannel architectures. The sensing mechanism was based on the nanochannel blockage caused by MS2 binding to immobilized capture antibodies. This blockage was quantified by measuring the oxidation current of the electroactive species reaching the electrode surface, by means of differential pulse voltammetry (DPV). The immunosensor showed a limit of detection of 6 pfu/mL in buffer, allowing the detection of MS2 to levels commonly found in real-world applications, and proved to be unaffected by matrix effects when analyzing MS2 in reservoir water. This platform enables the straightforward, direct and sensitive detection of a broad range of target analytes and constitutes a promising approach towards the development of portable electronic point of sample analysis devices.

References

Jun 26, 2008·Chembiochem : a European Journal of Chemical Biology·Nicolas H VoelckerM Reza Ghadiri
Nov 1, 2007·ACS Nano·Kristopher A KilianJ Justin Gooding
Mar 3, 2009·Trends in Biotechnology·Andrew JaneNicolas H Voelcker
Oct 20, 2010·Chemical Communications : Chem Comm·Alfredo de la Escosura-Muñiz, Arben Mekoçi
Mar 19, 2011·Parasitology·B J KingP T Monis
Jun 19, 2012·Biosensors & Bioelectronics·Alfredo de la Escosura-MuñizArben Merkoçi
Jul 6, 2012·Bioelectrochemistry·Binh Thi Thanh NguyenChee-Seng Toh
Aug 14, 2012·ACS Nano·Alfredo de la Escosura-Muñiz, Arben Merkoçi
Jul 23, 2013·Nature Immunology·Michael Reth
Jan 1, 2014·Analytical Chemistry·Beatriz Prieto-Simón, Josep Samitier
Jul 18, 2014·ACS Applied Materials & Interfaces·S N Aisyiyah JenieNicolas H Voelcker
Dec 11, 2014·ACS Applied Materials & Interfaces·Daniel BrodoceanuTobias Kraus

❮ Previous
Next ❯

Citations

Jun 22, 2019·Chemical Communications : Chem Comm·Maria AlbaNicolas H Voelcker
Jan 26, 2018·ACS Applied Materials & Interfaces·Nekane RetaNicolas H Voelcker

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.