Porous three-dimensional carbon nanotube scaffolds for tissue engineering

Journal of Biomedical Materials Research. Part a
Gaurav LalwaniBalaji Sitharaman

Abstract

Assembly of carbon nanomaterials into three-dimensional (3D) architectures is necessary to harness their unique physiochemical properties for tissue engineering and regenerative medicine applications. Herein, we report the fabrication and comprehensive cytocompatibility assessment of 3D chemically crosslinked macrosized (5-8 mm height and 4-6 mm diameter) porous carbon nanotube (CNT) scaffolds. Scaffolds prepared via radical initiated thermal crosslinking of single- or multiwalled CNTs (SWCNTs and MWCNTs) possess high porosity (>80%), and nano-, micro-, and macroscale interconnected pores. MC3T3 preosteoblast cells on MWCNT and SWCNT scaffolds showed good cell viability comparable to poly(lactic-co-glycolic) acid (PLGA) scaffolds after 5 days. Confocal live cell and immunofluorescence imaging showed that MC3T3 cells were metabolically active and could attach, proliferate, and infiltrate MWCNT and SWCNT scaffolds. SEM imaging corroborated cell attachment and spreading and suggested that cell morphology is governed by scaffold surface roughness. MC3T3 cells were elongated on scaffolds with high surface roughness (MWCNTs) and rounded on scaffolds with low surface roughness (SWCNTs). The surface roughness of scaffolds may be exploi...Continue Reading

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Citations

Jul 8, 2015·Journal of Biomedical Materials Research. Part a· HaryantoSeong Cheol Kim
Jun 23, 2016·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Carina AdamzykBernd Lethaus
Aug 17, 2016·Journal of Biomedical Materials Research. Part a·Gaurav LalwaniBalaji Sitharaman
Mar 16, 2017·Journal of Biomedical Materials Research. Part a·Gaurav LalwaniBalaji Sitharaman
Jan 13, 2019·Journal of Biomedical Materials Research. Part a·Behzad FarshidBalaji Sitharaman
Dec 23, 2015·Annals of Biomedical Engineering·Daniela Y SantiestebanStanislav Emelianov
Apr 30, 2016·Annals of Biomedical Engineering·Gaurav LalwaniBalaji Sitharaman
Jul 20, 2019·Journal of Biomaterials Science. Polymer Edition·Rafaella S S ZanetteMichele Munk
Sep 17, 2020·Materials·Tomasz SzymańskiMichael Giersig
Jan 6, 2021·Journal of Nanobiotechnology·Hamed NosratiZohreh Alizadeh

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