Porphyromonas gingivalis enhances FasL expression via up-regulation of NFkappaB-mediated gene transcription and induces apoptotic cell death in human gingival epithelial cells

Microbiology
Suzana BrozovicDenis F Kinane

Abstract

The interaction between epithelial cells and micro-organisms is often a crucial initiating event in infectious diseases. Infection with Porphyromonas gingivalis, a Gram-negative anaerobe, is strongly associated with severe periodontal disease. This bacterium possesses an array of virulence factors, some of which can induce apoptosis. The tumour necrosis factor (TNF) receptor family is involved in the regulation of cellular homeostasis, cell surface molecules involved in phagocytosis, Fas ligand (L) expression and activation of the caspase cascade resulting in DNA fragmentation and cell blebbing. The current study examined the role of nuclear factor-kappaB (NFkappaB) in FasL-mediated apoptotic cell death in primary human gingival epithelial cells (HGEC) induced by heat-killed P. gingivalis, probably through TLR signalling pathways. A marked up-regulation of TLR2 and Fas-FasL was detected in HGEC stimulated with P. gingivalis. Activation of NFkappaB by P. gingivalis in HGEC was demonstrated by an NFkappaB promoter luciferase assay as well as by phosphorylation of p65 as detected by Western blotting. Activation of cleaved caspase-3 and caspase-8 resulted in apoptotic cell death of HGEC. The survival proteins c-IAP-1/c-IAP-2 were d...Continue Reading

References

Apr 1, 1992·Journal of Periodontology·S S Socransky, A D Haffajee
Mar 1, 1984·Journal of Periodontal Research·E D Savitt, S S Socransky
Oct 1, 1994·Microbial Pathogenesis·Y Chen, A Zychlinsky
Mar 1, 1996·The Journal of Biological Chemistry·A M ChinnaiyanV M Dixit
Feb 7, 1997·Cell·S Nagata
Nov 1, 1996·Annals of Periodontology·S Offenbacher
Dec 9, 1997·Journal of Dental Research·F A RobertsS M Michalek
Feb 27, 1999·Journal of Cellular Physiology·P A Gregoli, M C Bondurant
Oct 3, 1999·European Journal of Immunology·S C HsuM Z Lai
Nov 15, 2000·Journal of Dental Research·J SandrosP N Papapanou
Jun 27, 2001·FEMS Microbiology Letters·S F NakhjiriR J Lamont
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 8, 2004·Microvascular Research·Shin-ichiro KuroshimaNobuo Inoue

❮ Previous
Next ❯

Citations

Jun 11, 2009·The Journal of Biological Chemistry·Manjunatha R BenakanakereDenis F Kinane
Mar 27, 2009·Journal of Virology·Penny ClarkeKenneth L Tyler
Mar 4, 2014·PloS One·Rupa BhattacharyaDana T Graves
Sep 26, 2012·Journal of Periodontology·Nawarat Wara-aswapatiYuichi Izumi
Dec 27, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hisanori DomonKazuhisa Yamazaki
Oct 28, 2008·European Journal of Cell Biology·Mingxia YuZhuoya Li
Apr 27, 2007·FEMS Microbiology Letters·Georg A RothEvanthia Lalla
Jan 6, 2019·Apoptosis : an International Journal on Programmed Cell Death·M R BenakanakereD F Kinane
Apr 24, 2019·Mediators of Inflammation·Paulo C Carvalho-FilhoSoraya C Trindade
Mar 29, 2020·Frontiers in Immunology·Yajie LiSinem Esra Sahingur

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis