Porphyromonas gingivalis ferrous iron transporter FeoB1 influences sensitivity to oxidative stress.

Infection and Immunity
Cecilia Anaya-BergmanJanina P Lewis

Abstract

Porphyromonas gingivalis FeoB1 is a ferrous iron transporter. Analysis of parental and feoB1-deficient strains of the periodontal pathogen revealed that the feoB1-deficient mutant strain had an increased ability to survive oxidative stress. Specifically, survival of the mutant strain was increased 33% with exposure to peroxide and 5% with exposure to atmospheric oxygen compared to the parental strain. Interestingly, the ability to survive intracellularly also increased fivefold in the case of the feoB1-deficient mutant. Our data suggest that although the FeoB1 protein is required for ferrous iron acquisition in P. gingivalis, it also has an adverse effect on survival of the bacterium under oxidative stress conditions. Finally, we show that feoB1 expression is not iron dependent and is dramatically reduced in the presence of host cells, consistent with the observed deleterious role it plays in bacterial survival.

References

Apr 1, 1995·Infection and Immunity·H M FletcherF L Macrina
Oct 1, 1993·Journal of Bacteriology·M KammlerK Hantke
Jan 6, 2000·Archives of Biochemistry and Biophysics·D Touati
Oct 29, 2000·Journal of Bacteriology·S G DashperE C Reynolds
Jan 12, 2001·Oral Microbiology and Immunology·N SlakeskiE C Reynolds
Apr 26, 2002·Molecular Microbiology·Maryta SztukowskaDonald M Kurtz
May 25, 2002·Journal of Bacteriology·David G KehresMichael E Maguire
Jun 28, 2003·FEMS Microbiology Reviews·Simon C AndrewsFrancisco Rodríguez-Quiñones
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Mar 24, 2005·Infection and Immunity·Yumiko Hosogi, Margaret J Duncan
May 20, 2005·The Journal of Biological Chemistry·Stuart G DashperEric C Reynolds
Jun 23, 2006·Infection and Immunity·Jia HeJanina P Lewis
Nov 6, 2007·Proteomics·Qiangwei XiaMurray Hackett

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Citations

Oct 14, 2010·The Journal of Biological Chemistry·Jin-Long GaoNeil Hunter
Apr 24, 2012·Molecular Oral Microbiology·S S YanamandraJ P Lewis
Nov 26, 2010·Antimicrobial Agents and Chemotherapy·Ji-Hoi MoonJin-Yong Lee
Mar 24, 2012·Future Microbiology·Leroy G HenryHansel M Fletcher
Jul 22, 2014·Molecular Oral Microbiology·C Anaya-BergmanJ P Lewis

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