PMID: 7516534Apr 1, 1994Paper

Porphyromonas gingivalis lipopolysaccharide stimulation of human monocytes: dependence on serum and CD14 receptor

Oral Microbiology and Immunology
L ShapiraT E Van Dyke

Abstract

The purpose of this study was to investigate factors influencing the ability of lipopolysaccharide (LPS) derived from Porphyromonas gingivalis to elicit secretion of tumor necrosis factor-alpha (TNF alpha) from human monocytes (adherent mononuclear cells). The results indicate that P. gingivalis LPS stimulation of TNF alpha from monocytes is comparable to LPS from Escherichia coli. Both LPS, although structurally different, increased TNF alpha secretion in a dose-dependent manner. In serum-free conditions, TNF alpha secretion was relatively low, but it dramatically increased at human serum concentrations as low as 1%. Maximal secretion was observed in the presence of 10% serum, with a slight decrease at higher serum concentrations. The CD14 molecule is a putative monocyte LPS receptor. When cells were pre-incubated with a blocking monoclonal antibody (My4) to CD14, TNF alpha-mRNA accumulation and TNF alpha secretion were reduced to control levels at LPS concentrations of up to 10 ng/ml. At higher LPS concentrations, the blocking effect was only partial, in spite of 50-fold excess antibody concentration. The blocking effect was observed only in the presence of serum. The effect of the CD14 antibody was dose-dependent with satura...Continue Reading

References

Jul 1, 1992·Immunology Today·W A Lynn, D T Golenbock
Apr 1, 1992·Journal of Periodontology·S S Socransky, A D Haffajee
May 1, 1991·Journal of Periodontal Research·R C Page
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·S L WeinsteinA L DeFranco
May 1, 1991·Journal of Periodontal Research·G R Mundy
Sep 15, 1991·Annals of Internal Medicine·R C Bone
Aug 1, 1991·Journal of Clinical Periodontology·P StashenkoS S Socransky
Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C R RaetzC F Nathan
Aug 1, 1991·Journal of Periodontology·P StashenkoS S Socransky
Jan 1, 1988·Journal of General Microbiology·D Drake, T C Montie
May 1, 1972·Journal of Periodontology·J SchwartzR B Parker
Sep 1, 1974·Journal of Periodontology·J J AleoA P Varboncoeur
Jan 1, 1983·Journal of Periodontal Research·B C NairE Hausmann

❮ Previous
Next ❯

Citations

Mar 27, 1999·Oral Microbiology and Immunology·T HiraokaT Sueda
Aug 1, 1996·Journal of Periodontal Research·M WilsonB Henderson
Sep 1, 2005·Journal of Clinical Periodontology·Lior ShapiraDenis F Kinane
Apr 27, 2004·Periodontology 2000·Douglas R DixonRichard P Darveau
Sep 14, 2007·Periodontology 2000·Denis F KinaneMichael H Martin
Jan 7, 2003·BMC Cell Biology·William L StoneMilton Smith
Dec 1, 2010·Immunity & Ageing : I & a·Yazdani B Shaik-DasthagirisahebFrank C Gibson
May 23, 2006·Metabolism: Clinical and Experimental·Jean-Claude MamputuGeneviève Renier
Aug 16, 2005·The International Journal of Biochemistry & Cell Biology·Yu-Jen ChenYun-Liang Chen
Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Jun 1, 1997·Periodontology 2000·R P DarveauR C Page
Jul 5, 2002·Journal of Dental Research·Y Houri-HaddadL Shapira
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P-L Wang, K Ohura
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E GemmellG J Seymour
Nov 22, 2001·Journal of Endotoxin Research·S AmarT Van Dyke
Apr 20, 2002·Current Opinion in Infectious Diseases·Dana T. GravesCaroline Genco
May 1, 2001·Journal of Periodontology·Lijian Jin, Richard P Darveau
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D CunninghamR P Darveau

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.