Abstract
To elucidate the genetic basis for the pronounced resistance that the oral pathogen, Porphyromonas gingivalis (P. gingivalis), exhibits towards the cationic antimicrobial peptide, polymyxin B. A genetic screen of P. gingivalis clones generated by a Tn4400'-based random insertion mutagenesis strategy was performed to identify bacteria harboring novel genetic mutations that render P. gingivalis susceptible to killing by the cationic antimicrobial peptide, polymyxin B (PMB, 50 microg x mL(-1)). P. gingivalis (ATCC 33277) is unusually resistant to the cationic antimicrobial peptide, PMB at relatively high concentrations (200 microg x mL(-1)). Approximately 2,700 independent Tn4400'-derived mutants of P. gingivalis were examined for increased sensitivity to PMB killing at a relatively low dose (50 microg x mL(-1)). A single PMB-sensitive mutant was obtained in this phenotypic screen. We determined that the Tn4400' transposon was integrated into the gene encoding the lipid A 4'-phosphatase, PGN_0524, demonstrating that this insertion event was responsible for its increased susceptibility of this clone to PMB-dependent killing. The resulting mutant strain, designated 0524-Tn4400', was highly sensitive to PMB killing relative to wild-t...Continue Reading
Citations
Aug 28, 2010·Biochemistry·Brian O IngramChristian R H Raetz
Jan 4, 2013·Glycobiology·Dusty B BrownRussell W Carlson
Jan 9, 2013·Clinical Microbiology Reviews·Jaroslav HrabákRadka Walková
May 1, 2015·Antibiotics·Victor I Band, David S Weiss
May 28, 2014·Mass Spectrometry Reviews·David J Harvey
Jan 13, 2015·Science·T W CullenA L Goodman
Apr 10, 2012·Peptides·Mahsa AbdolhosseiniSven-Ulrik Gorr
Dec 3, 2014·Regulatory Peptides·Denise T F McLeanFionnuala T Lundy
Dec 1, 2009·Journal of Zhejiang University. Science. B·Mei-ying ShaoTao Hu
Feb 2, 2015·Anaerobe·Leonor DíazDenisse Bravo
Jul 11, 2014·PLoS Pathogens·Connie SlocumCaroline A Genco
Nov 5, 2015·Journal of Periodontal Research·C SotoD Bravo
Jan 25, 2018·Journal of Applied Oral Science : Revista FOB·Alicia MoralesJorge Gamonal
Jun 21, 2017·Antimicrobial Agents and Chemotherapy·Adam J SanMiguelElizabeth A Grice
Jun 12, 2013·Nature Reviews. Microbiology·Brittany D Needham, M Stephen Trent
Jul 30, 2019·Frontiers in Cellular and Infection Microbiology·Katterinne N MendezDenisse Bravo
May 31, 2019·BMC Microbiology·Marlucy Rodrigues LimaAndrea de Souza Monteiro
Apr 8, 2020·Infection and Immunity·Nicole P GiordanoZachary D Dalebroux
Dec 19, 2018·Nature Microbiology·Bálint KintsesCsaba Pál
Mar 23, 2018·PloS One·Helmut HirtSven-Ulrik Gorr
Jul 13, 2021·Genomics, Proteomics & Bioinformatics·Zhenwei LiuXiaobing Wang