PMID: 9441858Mar 28, 1998Paper

Porphyromonas gingivalis virulence in a murine lesion model: effects of immune alterations

Microbial Pathogenesis
L KesavaluJ L Ebersole

Abstract

This study utilized various mouse strains with documented alterations in immune system components to assess their contribution to modify the virulence of Porphyromonas gingivalis. P. gingivalis W50 was cultivated on blood agar plates, harvested and used to challenge mice by subcutaneous injection on the dorsolateral surface of the back. Soft tissue lesion development was estimated by measuring the area of the spreading lesion formed by this microorganism over a period of 15 days. Challenge of various normal inbred and outbred mouse strains including: BALB/cN, BALB/cJ, BALB/c nu/+, ICR, B10.A(4R), B10.MBR, A/J, C57BL/6J, CBA/CaH, C.B-17/Icv Tacf DF and C3H/HeN with 2 x 10(10) bacteria showed similar lesion size among these strains (approximately 400 mm2). Genetically deficient mouse strains [C.B-17/Icr Tac (SCID); DBA/2 (C5 deficient); BALB/c nu/nu (T cell deficient); CBA/CaHN-XID/J (B cell deficient) and C3H/HeJ (LPS hyporesponsive)] demonstrated a lesion size which was similar to normal animals. C57BL/6J-BgJ (NK cell deficient) mice exhibited a significantly more severe lesion than the other strains tested. Following healing of the lesions, we initiated a secondary infection of the surviving animals to estimate the acquisition...Continue Reading

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Citations

Oct 16, 1999·Periodontology 2000·S C HoltC A Genco
Feb 19, 2011·Journal of Biomedicine & Biotechnology·Helieh S Oz, David A Puleo
Oct 2, 2014·BMC Microbiology·Kai BaoNagihan Bostanci
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May 26, 2007·Journal of Dental Research·Y Houri-HaddadL Shapira
Sep 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neil M O'Brien-SimpsonEric C Reynolds
Oct 4, 2002·Microbes and Infection·Pamela J Baker, Derry C Roopenian
Nov 18, 2021·Expert Review of Vaccines·Jihei Sara LeeKyoung Yul Seo

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