Portable and quantitative point-of-care monitoring of Escherichia coli O157:H7 using a personal glucose meter based on immunochromatographic assay

Biosensors & Bioelectronics
Haoran HuangWenchao Dou

Abstract

Here we innovate a portable and quantitative immunochromatographic assay (ICA) with a personal glucose meter (PGM) as readout for the detection of Escherichia coli O157:H7 (E. coli O157:H7). The carboxyl group coated Fe3O4 nanoparticles (MNPs) were synthesized by a one pot method and used as carriers of invertase and monoclonal antibody against E. coli O157:H7. Initially, the invertase and antibody double functionalized MNPs (Invertase-MNPs-IgG) conjugates were prepared and used as label probe in this assay system. Before laminating onto the baking card, the absorbent pad was soaked in sucrose solution and desiccated. MNPs produced brown band at the detection zone of the ICA when acting as direct labels. As they were also coupled with invertase, the invertase catalyzed the hydrolysis of sucrose on the absorbent pad into glucose, which was detected by the PGM. To increase the sensitivity, antibody functionalized MNPs were used to enrich E. coli O157:H7 from sample solution. The innovative aspect of this approach lies in the visualization and quantification of E. coli O157:H7 through Invertase-MNPs-IgG and the detection of glucose concentration using PGM. Although the feasibility is demonstrated using E. coli O157:H7 as a model a...Continue Reading

Citations

Nov 30, 2019·Advanced Materials·Jacob T HeggestadAshutosh Chilkoti
May 14, 2020·Diagnostics·Amanda MoyanoM Carmen Blanco-López
Oct 4, 2018·Analytical and Bioanalytical Chemistry·Linan ZhangLihua Li
Sep 16, 2020·Analytical Methods : Advancing Methods and Applications·Fang LiQinpu Zhang
Oct 6, 2020·SLAS Technology·Veronica R CampbellDaniel T Kamei
May 4, 2021·Talanta·Elham SheikhzadehMohammed Zourob
May 29, 2021·Critical Reviews in Food Science and Nutrition·Xiuguang XingWei Chen
May 23, 2021·International Journal of Food Microbiology·Alka RaniAndrew S Ball
Jul 7, 2021·Chemical Society Reviews·Erin M McConnellYingfu Li
Aug 29, 2018·Analytical Chemistry·Jun Ki AhnHyun Gyu Park
Feb 19, 2020·Analytical Chemistry·Niloufar AminArben Merkoçi
Jan 7, 2022·Journal of Agricultural and Food Chemistry·Leina DouZhanhui Wang

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