Abstract
In a pig model of intestinal transplantation, we previously showed that hepatic conditioning through portal donor-specific blood transfusion (pDSBT), high-dose tacrolimus (TAC), and steroids prevented rejection and increased survival Our current study tests a protocol of pDSBT, short-term mycophenolate mofetil (MMF), and low-dose TAC to eliminate the use of steroids, reduce TAC dosage, and increase the level of chimerism in the peripheral blood. Four groups of outbred, mixed lymphocyte culture (MLC)-reactive pigs underwent bowel transplants and pDSBT. Immunosuppression (group 1, high-dose TAC and steroids; group 2, low-dose TAC and MMF; group 3, low-dose TAC, MMF, and aminoguanidine; group 4, low-dose TAC, MMF, and arginine) was discontinued after 28 days. RNA was extracted from intestinal graft and native liver biopsies for cytokine measurements. Chimerism levels were determined using a Q-PCR analysis. Pig survival and death rates due to rejection did not significantly differ between the four groups. Chimerism levels determined by Q-PCR analysis were not different until day 28. After discontinuation of immunosuppression, we noted a trend (P = 0.15) toward higher mean chimerism levels on day 60 for groups 2, 3, and 4 (9%) vs. g...Continue Reading
References
May 1, 1991·Transplantation·M P CalleryM W Flye
May 1, 1990·The Journal of Surgical Research·T KameiM W Flye
Nov 11, 1988·Nucleic Acids Research·R A McGrawM Akamatsu
Aug 12, 1967·Nature·H M Cantor, A E Dumont
Nov 27, 1995·Transplantation·P AlardJ W Streilein
Mar 16, 1993·European Journal of Pharmacology·T P MiskoM G Currie
Apr 1, 1993·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Y NakafusaM W Flye
Jul 1, 1996·The Journal of Surgical Research·K SatoT Maekawa
Apr 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H TashiroK Dohi
Jul 1, 1996·The Annals of Thoracic Surgery·P MattssonB P Griffith
Aug 1, 1996·The Annals of Thoracic Surgery·N K WorrallT B Ferguson
Jan 1, 1996·Transplant International : Official Journal of the European Society for Organ Transplantation·R E NakhlehR W Gruessner
Jan 15, 1997·Transplantation·R W GruessnerA C Gruessner
Jan 1, 1997·Surgery·J PirenneR W Gruessner
Apr 27, 1997·Transplantation·N K WorrallG A Patterson
Aug 13, 1998·Transplantation·R W GruessnerA C Gruessner
Mar 20, 1999·Transplantation Proceedings·J M LangrehrP J Neuhaus
Sep 21, 2000·Transplantation Proceedings·N MorinP Metrakos
Mar 27, 2001·Transplantation Proceedings·T KoshibaJ Pirenne
Aug 4, 2001·Archives of Surgery·J V HarmonR W Gruessner
Aug 29, 2001·Annals of Surgery·K Abu-ElmagdT E Starzl
Mar 30, 2002·Transplantation·Jacques PirenneMark Waer
Jul 23, 2002·Transplantation·Tao WuZhiguang Guo
Jul 5, 2003·The Journal of Surgical Research·Hitoshi KimuraKoichi Hirata
Citations
May 23, 2009·Transplantation·Bianca KruseMartin Strüber
Apr 9, 2005·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J A Diaz-Peromingo, A Gonzalez-Quintela
Sep 24, 2004·Transplant Immunology·Jacques Pirenne, Masaru Kawai
Nov 26, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M AvsarG Warnecke
Jul 30, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dimitra KotsougianiAllen T Bishop
Jan 18, 2017·Transplant International : Official Journal of the European Society for Organ Transplantation·Wiebke SommerGregor Warnecke
Oct 11, 2016·Cellular & Molecular Immunology·Rita DiehlJohannes Boltze
Jul 15, 2017·Transplantation Direct·Katharina JanssonGregor Warnecke
Jul 10, 2019·Stem Cells and Development·Jesus M Sierra-ParragaMarco Eijken
Mar 6, 2018·Microsurgery·Dimitra KotsougianiAllen T Bishop
Sep 22, 2020·Transplantation·Stine LohmannBente Jespersen
Nov 1, 2020·Transplant Immunology·Karolin S HackerGregor Warnecke