Portal venous hyperinsulinemia does not stimulate gut glucose absorption in the conscious dog

Metabolism: Clinical and Experimental
Raul C CamachoD H Wasserman

Abstract

The purpose of the present study was to assess whether physiological portal vein hyperinsulinemia stimulates gut glucose absorption in vivo. Chronically catheterized (femoral artery, portal vein, inferior vena cava, and proximal and distal duodenum) and instrumented (Doppler flow probe on portal vein) insulin (INS, 2 mU.kg(-1).min(-1), n = 6) or saline (SAL, n = 5) infused dogs were studied during basal (30 minutes) and experimental (90 minutes) periods. Arterial and portal vein plasma insulin were 3.3- and 3.2-fold higher, respectively, throughout the study in INS compared to SAL. An intraduodenal glucose infusion of 8 mg.kg(-1).min(-1) was initiated at t = 0 minutes. At t = 20 and 80 minutes, a bolus of 3-O-[3H]methylglucose (MG) and L-[14C]glucose (L-GLC) was injected intraduodenally. Phloridzin, an inhibitor of the Na+ -dependent glucose transporter (SGLT1), was infused from t = 60 to 90 minutes in the presence of a peripheral isoglycemic clamp. Net gut glucose output (NGGO) was 5.2 +/- 0.6 and 4.6 +/- 0.8 mg.kg(-1).min(-1) in INS and SAL, respectively, from t = 20 to 60 minutes. Transporter-mediated absorption was 87% +/- 2% of NGGO in both INS and SAL. Passive gut glucose absorption was 13% +/- 2% of NGGO in both INS and ...Continue Reading

References

Aug 1, 1991·Canadian Journal of Physiology and Pharmacology·R N FedorakV M Porter
Jun 1, 1995·The Journal of Clinical Investigation·M R Uhing, R E Kimura
Jun 1, 1995·The Journal of Clinical Investigation·M R Uhing, R E Kimura
May 13, 2003·Journal of Applied Physiology·R Richard PencekDavid H Wasserman

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Citations

Jul 25, 2008·Diabetes Care·Mary E PennantDavid B Dunger

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