Portrait of transcriptional responses to ultraviolet and ionizing radiation in human cells

Nucleic Acids Research
Kerri E Rieger, Gilbert Chu

Abstract

To understand the human response to DNA damage, we used microarrays to measure transcriptional responses of 10 000 genes to ionizing radiation (IR) and ultraviolet radiation (UV). To identify bona fide responses, we used cell lines from 15 individuals and a rigorous statistical method, Significance Analysis of Microarrays (SAM). By exploring how sample number affects SAM, we rendered a portrait of the human damage response with a degree of accuracy unmatched by previous studies. By showing how SAM can be used to estimate the total number of responsive genes, we discovered that 24% of all genes respond to IR and 32% respond to UV, although most responses were less than 2-fold. Many genes were involved in known damage-response pathways for cell cycling and proliferation, apoptosis, DNA repair or the stress response. However, the majority of genes were involved in unexpected pathways, with functions in signal transduction, RNA binding and editing, protein synthesis and degradation, energy metabolism, metabolism of macromolecular precursors, cell structure and adhesion, vesicle transport, or lysosomal metabolism. Although these functions were not previously associated with the damage response in mammals, many were conserved in yeas...Continue Reading

References

Jun 13, 1998·The Journal of Biological Chemistry·B P LipskyD E Staunton
Jun 23, 1998·The Journal of Biological Chemistry·M S IordanovB E Magun
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·N Li, M Karin
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Apr 25, 2000·Molecular and Cellular Biology·C BlattnerP Herrlich
Dec 5, 2000·Molecular Biology of the Cell·A P GaschP O Brown
Mar 10, 2001·International Journal of Radiation Oncology, Biology, Physics·L LiR J Legerski
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 13, 2001·Oncogene·E Shaulian, M Karin
Oct 18, 2001·Radiation Research·B N FordB L Tracy
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·G W BirrellJ M Brown
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D LiM Blumenberg
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Angela SestoJosé L Jorcano
Apr 9, 2002·Experimental Hematology·Xiaoxia Li, George R Stark
May 4, 2002·Nature Cell Biology·Eitan Shaulian, Michael Karin
Jun 18, 2002·The EMBO Journal·Lisa G DeFazioGilbert Chu
Jun 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·J HohJ Ott
Jun 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Geoff W BirrellJ Martin Brown
Aug 31, 2002·Environmental and Molecular Mutagenesis·Yueliang Leon GuoWenya Huang
Nov 28, 2002·Molecular Cell·Reuben S HarrisMichael S Neuberger
Dec 6, 2002·Oncogene·Woong-Yang ParkJeong-Sun Seo
Jan 17, 2003·Molecular Biology of the Cell·Dongrong ChenJürg Bähler
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kerri E RiegerGilbert Chu

❮ Previous
Next ❯

Citations

Feb 11, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Theres LindgrenDavid Eriksson
May 12, 2005·Mutation Research·Timothy W Gant, Shu-Dong Zhang
Mar 16, 2005·Nucleic Acids Research·Emily E Bosco, Erik S Knudsen
Oct 15, 2009·Nucleic Acids Research·Wan-Jen HongGilbert Chu
Feb 28, 2009·Genome Research·Curtis HuttenhowerOlga G Troyanskaya
Sep 22, 2007·Acta Pharmacologica Sinica·Hai-Ying ChenWen-Gong Yu
Oct 25, 2011·Photochemistry and Photobiology·Patricia CramersAlbert A van Zeeland
Apr 6, 2006·Genome Biology·Martino BarencoMichael Hubank
May 10, 2007·PloS One·Lilian RicaudMarie-Hélène Montané
Nov 1, 2011·PloS One·Carl N SprungHelen B Forrester
Aug 1, 2013·International Journal of Molecular Sciences·Mykyta Sokolov, Ronald Neumann
Mar 13, 2014·International Journal of Genomics·Alessandra TessitoreEdoardo Alesse
Nov 4, 2015·Biomolecules·Lulzim Shkreta, Benoit Chabot
Oct 30, 2015·International Journal of Molecular Sciences·Nikolett SándorHargita Hegyesi
Feb 20, 2016·Molecular Cell·Vihandha O Wickramasinghe, Ashok R Venkitaraman
May 9, 2008·International Journal of Radiation Biology·A TurtoiF H A Schneeweiss
Mar 17, 2010·FEBS Letters·Aziz SancarNuri Ozturk
Jan 19, 2011·Cardiovascular Revascularization Medicine : Including Molecular Interventions·Yunus AyralKlaus Pels
Oct 30, 2010·Journal of Dermatological Science·Young-Sook LeeJeung-Hoon Lee
Jan 14, 2009·Molecular and Biochemical Parasitology·Christian WeberCésar López-Camarillo
Mar 8, 2008·Journal of Dermatological Science·Young-Sook LeeJeung-Hoon Lee
Jun 15, 2007·Free Radical Biology & Medicine·Mikael MolinJean Labarre
Oct 31, 2006·International Journal of Radiation Oncology, Biology, Physics·Enikö KisGéza Sáfrány
Jan 18, 2007·The Plant Journal : for Cell and Molecular Biology·Kevin M CulliganAnne B Britt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis