Position-dependent interactions of Y-box protein 2 (YBX2) with mRNA enable mRNA storage in round spermatids by repressing mRNA translation and blocking translation-dependent mRNA decay

Molecular Reproduction and Development
K C Kleene

Abstract

Many mRNAs encoding proteins needed for the construction of the specialized organelles of spermatozoa are stored as translationally repressed, free messenger ribonucleoproteins in round spermatids, to be actively translated in elongating and elongated spermatids. The factors that repress translation in round spermatids, however, have been elusive. Two lines of evidence implicate the highly abundant and well-known translational repressor, Y-box protein 2 (YBX2), as a critical factor: First, protamine 1 (Prm1) and sperm-mitochondria cysteine-rich protein (Smcp) mRNAs are prematurely recruited onto polysomes in Ybx2-knockout mouse round spermatids. Second, mutations in 3' untranslated region (3'UTR) cis-elements that abrogate YBX2 binding activate translation of Prm1 and Smcp mRNAs in round spermatids of transgenic mice. The abundance of YBX2 and its affinity for variable sequences, however, raise questions of how YBX2 targets specific mRNAs for repression. Mutations to the Prm1 and Smcp mRNAs in transgenic mice reveal that strong repression in round spermatids requires YBX2 binding sites located near the 3' ends of their 3'UTRs as locating the same sites in upstream positions produce negligible repression. This location-dependenc...Continue Reading

References

May 1, 1975·The Journal of Cell Biology·A L Kierszenbaum, L L Tres
Jan 1, 1991·Annals of the New York Academy of Sciences·R Oliva, G H Dixon
Jan 1, 1989·Reproduction, Fertility, and Development·P MaliM Parvinen
Jul 15, 1994·Biochemical and Biophysical Research Communications·K NayerniaW Engel
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SchindelinU Heinemann
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·K LeeR E Braun
Aug 15, 1998·Trends in Cell Biology·K Matsumoto, A P Wolffe
Apr 17, 1999·Molecular and Cellular Biology·E E SchmidtM R Capecchi
Sep 22, 2001·Biology of Reproduction·J YuR M Schultz
Feb 20, 2002·Journal of Molecular Biology·Cathelijne P A M KloksCornelis W Hilbers
May 14, 2003·Chromosoma·Marvin L MeistrichMing Zhao
Jun 6, 2003·Biochemical and Biophysical Research Communications·Ken MatsumotoMasafumi Tsujimoto
Aug 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wei YanMartin M Matzuk
Mar 9, 2004·Nature Structural & Molecular Biology·Caterina AlfanoMaria R Conte
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chon-Hwa Tsai-MorrisMaria L Dufau

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Citations

Aug 22, 2018·Systems Biology in Reproductive Medicine·Kenneth Cole Kleene
Apr 29, 2019·BMC Genomics·Nedenia Bonvino StafuzzaDaniela A Lino Lourenco
Nov 6, 2020·Cell and Tissue Research·Bala Krishnan BinsilaRaghavendra Bhatta
Nov 9, 2021·Cellular and Molecular Life Sciences : CMLS·Marcos MorganMarine Baptissart

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