Position-dependent plasticity of distinct progenitor types in the primitive streak

ELife
Filip J WymeerschValerie Wilson

Abstract

The rostrocaudal (head-to-tail) axis is supplied by populations of progenitors at the caudal end of the embryo. Despite recent advances characterising one of these populations, the neuromesodermal progenitors, their nature and relationship to other populations remains unclear. Here we show that neuromesodermal progenitors are a single Sox2(low)T(low) entity whose choice of neural or mesodermal fate is dictated by their position in the progenitor region. The choice of mesoderm fate is Wnt/β-catenin dependent. Wnt/β-catenin signalling is also required for a previously unrecognised phase of progenitor expansion during mid-trunk formation. Lateral/ventral mesoderm progenitors represent a distinct committed state that is unable to differentiate to neural fates, even upon overexpression of the neural transcription factor Sox2. They do not require Wnt/β-catenin signalling for mesoderm differentiation. This information aids the correct interpretation of in vivo genetic studies and the development of in vitro protocols for generating physiologically-relevant cell populations of clinical interest.

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Citations

Mar 30, 2018·ELife·Oriol Viader-LlarguésHernán López-Schier
Apr 11, 2018·Development·Beatriz López-EscobarPatricia Ybot-González
Jun 22, 2017·Development·Jérome Chal, Olivier Pourquié
Aug 11, 2018·ELife·Thomas Jr FrithAnestis Tsakiridis
Dec 20, 2017·ELife·Andrea CorsinottiIan Chambers
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Apr 27, 2019·Development·Shlomit EdriAlfonso Martinez Arias
Sep 29, 2019·Cellular and Molecular Life Sciences : CMLS·Moisés Mallo
Sep 2, 2017·Genes & Development·Jacqueline Deschamps, Denis Duboule
Nov 11, 2018·Development·David R McClayStacy L Feinberg
Mar 8, 2016·ELife·Aida Rodrigo Albors, Kate G Storey
Jul 31, 2020·Nature·Stefanie GrosswendtAlexander Meissner
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Feb 2, 2021·Frontiers in Cell and Developmental Biology·Ramkumar Sambasivan, Benjamin Steventon
Feb 13, 2021·International Journal of Molecular Sciences·Shoichiro TaniHironori Hojo
Feb 18, 2021·Development·Filip J WymeerschAnestis Tsakiridis
Oct 28, 2020·Development·Manuel RochaVictoria E Prince
Feb 20, 2021·Stem Cell Reports·Stefania CarobbioAntonio Vidal-Puig
Mar 8, 2021·Current Opinion in Neurobiology·Stéphane Nedelec, Alfonso Martinez-Arias
Apr 3, 2021·Cellular and Molecular Life Sciences : CMLS·Karolina PunovuoriSally Lowell

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Methods Mentioned

BETA
transgenic
PCR
dissecting

Software Mentioned

Prism
Icy
GB
ImageJ
Adobe Photoshop
Huygens
Volocity
FARSIGHT

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