Position effect on expression of dsd genes cloned onto multicopy plasmids.

Journal of Bacteriology
A M CarothersE McFall

Abstract

In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid carrying the regulatory gene, dsdC+, did not promote expression of chromosomal dsdCO+A+ loci, nor did a chromosomal dsdC+ gene promote expression of plasmid-borne dsdC delta O+A+ (dsd regulatory gene negative) restriction fragments. However, hybrid plasmids that comprise the entire dsd system (dsdC+O+A+) are highly inducible for the enzyme. These dsd hybrid plasmid deoxyribonucleic acids functioned well as templates in the in vitro coupled transcription-translation system. In vitro-synthesized dsdC+ protein promoted expression of the dsdA+ operation efficiently. Exogenously purified dsdC+ protein also activated expression of several dsdC delta O+A+ plasmid deoxyribonucleic acid templates in vitro. An explanation that reconciles these results with previous dominance studies is presented.

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G M McCorkleH E Umbarger
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M C HeinczE McFall
Jun 20, 1979·Molecular & General Genetics : MGG·L J Wallace, G Wilcox
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K BackmanW Gilbert
Mar 1, 1975·Journal of Bacteriology·F R BloomA M Carothers
Jan 1, 1973·Annual Review of Genetics·G Zubay
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·V HershfieldD R Helinski

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Citations

Oct 1, 1984·Journal of Bacteriology·M C HeinczE McFall
Aug 1, 1986·Journal of Bacteriology·E McFall

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