Positional cloning of a Bombyx pink-eyed white egg locus reveals the major role of cardinal in ommochrome synthesis

Heredity
Mizuko Osanai-FutahashiH Sezutsu

Abstract

Ommochromes are major insect pigments involved in coloration of compound eyes, eggs, epidermis and wings. In the silkworm Bombyx mori, adult compound eyes and eggs contain a mixture of the ommochrome pigments such as ommin and xanthommatin. Here, we identified the gene involved in ommochrome biosynthesis by positional cloning of B. mori egg and eye color mutant pink-eyed white egg (pe). The recessive homozygote of pe has bright red eyes and white or pale pink eggs instead of a normal dark coloration due to the decrease of dark ommochrome pigments. By genetic linkage analysis, we narrowed down the pe-linked region to ~258 kb, containing 17 predicted genes. RNA sequencing analyses showed that the expression of one candidate gene, the ortholog of Drosophila haem peroxidase cardinal, coincided with egg pigmentation timing, similar to other ommochrome-related genes such as Bm-scarlet and Bm-re. In two pe strains, a common missense mutation was found within a conserved motif of B. mori cardinal homolog (Bm-cardinal). RNA interference-mediated knockdown and transcription activator-like effector nuclease (TALEN)-mediated knockout of the Bm-cardinal gene produced the same phenotype as pe in terms of egg, adult eye and larval epidermis c...Continue Reading

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Citations

Oct 11, 2016·Current Opinion in Insect Science·Ryo Futahashi
Oct 11, 2016·Current Opinion in Insect Science·Haruhiko Fujiwara, Hideki Nishikawa
Jul 11, 2018·Biological Reviews of the Cambridge Philosophical Society·Florent Figon, Jérôme Casas
Jan 13, 2018·PLoS Genetics·Carolyn A MorrisonJessica E Treisman
Dec 12, 2018·PLoS Computational Biology·Aleksandr V ZhuravlevElena V Savvateeva-Popova
Dec 28, 2018·Archives of Insect Biochemistry and Physiology·Colin S Brent, J Joe Hull
Jul 25, 2020·Biochemical and Biophysical Research Communications·Yu Shirai, Takaaki Daimon
Jun 24, 2020·Insect Biochemistry and Molecular Biology·Florent FigonJérôme Casas
May 25, 2021·Current Opinion in Genetics & Development·Xiaoling TongSongyuan Wu

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