Positional determination of the naso-temporal retinal axis coincides with asymmetric expression of proteins along the anterior-posterior axis of the eye primordium

Experimental Eye Research
S ThanosE Hummler

Abstract

We used two different methodologies to examine at what stage development retinal positional specificity is established and which molecules are responsible. The first goal was achieved by removing parts of the presumptive temporal primary optic vesicle at stage 11 (40 to 45 hr of incubation) and fate mapping of tissue with presumptive nasal properties that shifted into the wound during the events of wound-healing. Participation of the shifted tissue in the healing resulted in assembly of a temporal retina with mosaic-like projection properties, as examined by retrograde double staining of the retinal ganglion cells from the optic tectum. In addition to cells with normal temporal-rostral projections, clusters of ganglion cells with nasal-like projection identities appeared labelled within the temporal hemiretina. The number of clusters increased with the amount of resected tissue, and by almost complete ablation of the presumptive temporal anlage, a temporal hemiretina with predominantly nasal retinotectal specificity was created. These neuroanatomical results suggested that neuroepithelial cells had fixed nasal and temporal positional specificities at stage 11. To examine differences in the cells derived of either half of the ey...Continue Reading

Citations

Nov 27, 2009·BMC Developmental Biology·Sae Kyung Shin, Keely M Bumsted O'Brien
Dec 10, 2008·The Journal of Comparative Neurology·Ana Pombero, Salvador Martinez
Jun 26, 2001·Brain Research. Brain Research Reviews·S Thanos, J Mey
Apr 13, 2000·Brain Research. Brain Research Reviews·J Mey, S Thanos

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