Positional effects of the matrix attachment region on transgene expression in stably transfected CHO cells

Cell Biology International
Tianyun WangJun-Tang Lin

Abstract

Previous work has shown that the MAR (matrix attachment region) could increase transgene expression in stably transfected CHO (Chinese-hamster ovary) cells. To study the positional effect of MAR on transgene expression, three expression vectors were constructed which contained the human beta-globin MAR in different sites, including the vector with two MARs flanking the CAT (chloramphenicol acetyltransferase) expression cassette, one MAR at the 59 or 39 site. These vectors were transfected into CHO cells. The level of CAT gene expression was most effectively increased by two MARs flanking the CAT expression cassette. This increase was also seen when MAR was inserted at the 59 site upstream of the expression cassette, whereas the transgene expression level decreased when MAR was inserted at the 39 site downstream of the expression cassette. We have also shown that the transgene expression level is not directly proportional to the gene copy number, and gene copy number dependency does not exist.

References

Apr 1, 1992·Current Opinion in Genetics & Development·U K LaemmliY Adachi
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R A McKnightL Hennighausen
Jan 9, 2004·Journal of Biotechnology·Jong-Mook KimYeup Yoon
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Katleen M J ButayeMiguel F C De Bolle
Dec 15, 2006·Plant Biotechnology Journal·Apolonia H M Van der GeestWilliam M Ainley
Jul 6, 2007·Applied Microbiology and Biotechnology·Tianyun WangYafeng Wang
Aug 16, 2008·Cell Biology International·Tian-Yun WangXian-Jun Yang

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Citations

Jun 20, 2012·Biotechnology and Bioengineering·William Pooi Kat ChongYing Swan Ho
Apr 17, 2013·Molecular Medicine Reports·Qin LiLi Wang
Oct 28, 2017·Journal of Cellular and Molecular Medicine·Zheng-Wei TianGuang-Hua Xu

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