PMID: 7334294Oct 1, 1981Paper

Positioning of inner cell mass determines the development of mouse blastocysts in vitro

Journal of Embryology and Experimental Morphology
T C WuI Damjanov

Abstract

We have explanted mouse blastocysts into culture dishes and studies the positioning of the inner cell mass (ICM) at the time of attachment in order to establish what effect the location of the inner cell mass has on the subsequent development of embryos in vitro. We show that blastocysts may attach to the substrate with any portion of the trophectoderm. The location of ICM within the attaching blastocysts is unpredictable. The directions of egg-cylinder development may be either upward or downward. The axis assumed by a developing egg cylinder in vitro depends to a large extent on the initial positioning of the ICM at the time of attachment. Egg cylinders grown from blastocysts that have the ICM in the lower position (close to the plastic dish) reach advanced stages of development in greater numbers than egg cylinders grown from blastocyst with their ICM's in the upper lateral or upper position. Only upward-growing egg cylinders can develop further into somitic stage (which is equivalent to 8 1/2- or 9-day in vivo embryos). In contrast, downward growing egg cylinders become thwarted in their further development by the surrounding trophectoderm. If the downward growing egg cylinders are freed of trophectoderm and repositioned to...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.