PMID: 8593679Nov 1, 1995Paper

Positive and negative elements involved in the differential regulation by heme and oxygen of the HEM13 gene (coproporphyrinogen oxidase) in Saccharomyces cerevisiae

Current Genetics
J M AmilletR Labbe-Bois

Abstract

The Saccharomyces cerevisiae HEM13 gene codes for coproporphyrinogen oxidase (CPO), an oxygen-requiring enzyme catalysing the sixth step of heme biosynthesis. Its transcription is increased 40-50-fold in response to oxygen- or heme-deficiency. We have analyzed CPO activity and HEM13 mRNA levels in a set of isogenic strains carrying single or double deletions of the CYP1 (HAP1), ROX1, SSN6, or TUP1 genes. The cells were grown in the presence or absence of oxygen and under heme-deficiency (hem1 delta background). Both Rox1p and Cyp1p partially repressed HEM13 in aerobic heme-sufficient cells, probably in an independent manner. In the absence of heme, Cyp1p activated HEM13 and strongly repressed ROX1, allowing de-repression of HEM13. Cyp1p had no effect on HEM13 expression in anaerobic cells. Deletions of SSN6 or TUP1 dramatically de-repressed HEM13 in aerobic cells. A series of deletions in the HEM13 promoter identified at least four regulatory regions that are required for HEM13 regulation. Two regions, containing motifs similar to the Rox1p consensus sequences, act as repression sites under aerobic growth. The two other sites act as activation sequences required for full induction under oxygen- or heme-deficiency. Taken togethe...Continue Reading

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Citations

Oct 12, 2013·Applied Microbiology and Biotechnology·Angelique C W FrankenPeter J Punt
Mar 30, 2010·FEMS Yeast Research·Raquel Castro-PregoMaría Esperanza Cerdán
Feb 1, 1997·Kidney International·R S ZitomerJ Deckert
Aug 1, 1997·Yeast·M González-DomínguezM E Cerdán
Oct 7, 2005·Microbiology·Chengshu WangRaymond J St Leger
May 28, 2005·Canadian Journal of Microbiology·Moisés BlancoM Esperanza Cerdán
Dec 2, 1998·Genetics·J DeckertR S Zitomer

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