PMID: 9537375Apr 16, 1998Paper

Positive and negative transcriptional regulation of the Escherichia coli gluconate regulon gene gntT by GntR and the cyclic AMP (cAMP)-cAMP receptor protein complex

Journal of Bacteriology
N Peekhaus, T Conway

Abstract

The gntT gene of Escherichia coli is specifically induced by gluconate and repressed via catabolite repression. Thus, gluconate is both an inducer and a repressor of gntT expression since gluconate is a catabolite-repressing sugar. In a gntR deletion mutant, the expression of a chromosomal gntT::lacZ fusion is both high and constitutive, confirming that GntR is the negative regulator of gntT. Indeed, GntR binds to two consensus gnt operator sites; one overlaps the -10 region of the gntT promoter, and the other is centered at +120 with respect to the transcriptional start site. The binding of GntR to these sites was proven in vitro by gel redardation assays and in vivo by site-directed mutagenesis of the binding sites. Binding of GntR to the operators is eliminated by gluconate and also by 6-phosphogluconate at a 10-fold-higher concentration. Interestingly, when gntR deletion strains are grown in the presence of gluconate, there is a twofold decrease in gntT expression which is independent of catabolite repression and binding of GntR to the operator sites. This novel response of gntR mutants to the inducer is termed ultrarepression. Transcription of gntT is activated by binding of the cyclic AMP (cAMP)-cAMP receptor protein (CRP...Continue Reading

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Citations

Oct 6, 2000·Annual Review of Microbiology·M Riley, M H Serres
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Erik van NimwegenEric D Siggia
Oct 28, 2006·Nucleic Acids Research·Andrew D S Cameron, Rosemary J Redfield
Jan 8, 2013·Journal of Bacteriology·Axel Cournac, Jacqueline Plumbridge
Nov 2, 2004·Journal of Bacteriology·Cristina Bates UtzTyrrell Conway
Oct 27, 2015·Applied Microbiology and Biotechnology·Koichi Toyoda, Masayuki Inui
Jan 22, 2005·Journal of Bacteriology·Elizabeth L Murray, Tyrrell Conway
Jan 29, 2005·Journal of Basic Microbiology·Ali M ElshafeiLatifa A Mohamed
Jun 30, 2001·Evolution; International Journal of Organic Evolution·I N Wang, D E Dykhuizen
Jul 4, 2012·The Journal of Microbiology·Yu-Pei ChenChing-Ping Tseng
Jan 19, 2016·Bioprocess and Biosystems Engineering·Young Hoon OhSi Jae Park
Jul 11, 1998·Journal of Bacteriology·N Peekhaus, T Conway
Dec 1, 2018·Nature Communications·Sebastian KnorrJörg S Hartig
Jul 29, 2017·Environmental Microbiology·A DaddaouaTino Krell
Dec 1, 2004·EcoSal Plus·M-A Mandrand-BerthelotN Hugouvieux-Cotte-Pattat
Nov 1, 2005·EcoSal Plus·Tony Romeo, Jacky L Snoep
Sep 2, 2008·Journal of Molecular Biology·Andrew D S Cameron, Rosemary J Redfield

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