Positive autoregulation of mrkHI by the cyclic di-GMP-dependent MrkH protein in the biofilm regulatory circuit of Klebsiella pneumoniae

Journal of Bacteriology
Jason W H TanRichard A Strugnell

Abstract

Klebsiella pneumoniae is an important cause of nosocomial infections, primarily through the formation of surface-associated biofilms to promote microbial colonization on host tissues. Expression of type 3 fimbriae by K. pneumoniae facilitates surface adherence, a process strongly activated by the cyclic di-GMP (c-di-GMP)-dependent transcriptional activator MrkH. In this study, we demonstrated the critical importance of MrkH in facilitating K. pneumoniae attachment on a variety of medically relevant materials and demonstrated the mechanism by which bacteria activate expression of type 3 fimbriae to colonize these materials. Sequence analysis revealed a putative MrkH recognition DNA sequence ("MrkH box"; TATCAA) located in the regulatory region of the mrkHI operon. Mutational analysis, electrophoretic mobility shift assay, and quantitative PCR experiments demonstrated that MrkH binds to the cognate DNA sequence to autoregulate mrkHI expression in a c-di-GMP-dependent manner. A half-turn deletion, but not a full-turn deletion, between the MrkH box and the -35 promoter element rendered MrkH ineffective in activating mrkHI expression, implying that a direct interaction between MrkH and RNA polymerase exists. In vivo analyses showed ...Continue Reading

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Citations

Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Maria A Schumacher, Wenjie Zeng
Sep 22, 2016·Protein & Cell·Feng WangLichuan Gu
Apr 5, 2018·Journal of Innate Immunity·Sebastian A RiquelmeAlice Prince
Apr 5, 2017·The Journal of Infectious Diseases·Angela Gomez-Simmonds, Anne-Catrin Uhlemann
Apr 1, 2020·Journal of Medical Microbiology·Lijiang ChenTieli Zhou
Sep 16, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Martha Lorena Ostria-HernandezJorge E Vidal

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