Positive correlation between allelic loss at chromosome 14q24-31 and poor prognosis of patients with renal cell carcinoma

Urology
Haruki KakuKenji Shimizu

Abstract

To report our development of a new application of the inter-Alu long polymerase chain reaction (PCR) for genomic scanning to screen for tumor-specific alterations in tumor DNA. Using this method, we detected a rearranged chromosomal region in renal cell carcinomas (RCCs). We then examined tumor-specific allelic loss in this region using microsatellite markers and determined whether a relationship was present between this allelic loss and the clinicopathologic features of the patients. The inter-Alu long PCR genomic scan method was performed using RCC DNA samples and primers specific for a minor subset of the human repeat sequence Alu. We analyzed DNA samples from 42 pairs of matched normal and nonpapillary RCC tissues with seven microsatellite markers. The inter-Alu long PCR genomic scan method revealed an altered DNA region on chromosome 14q24-31, which is the location of several putative tumor suppressor genes. At least one of seven microsatellite markers on chromosome 14q24-31 showed loss of heterozygosity in 23 (54.8%) of 42 informative cases of RCC. The prevalent loss region was confined to a 2-Mb region around D14S67. We found a positive correlation between the presence of the loss of heterozygosity on 14q24-31 and tumor ...Continue Reading

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Citations

Jul 4, 2012·Seminars in Cancer Biology·Eamonn R Maher
May 25, 2005·International Journal of Cancer. Journal International Du Cancer·Tapasya SrivastavaSubrata Sinha
Jun 11, 2009·Journal of Cellular and Molecular Medicine·Arnab PalParthaprasad Chattopadhyay
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Jan 8, 2019·The Journal of Clinical Investigation·John C ChappellW Kimryn Rathmell
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Eri AraiYae Kanai

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