PMID: 11903384Mar 21, 2002Paper

Positive effect on T-cell regulatory apoptosis by mycophenolate mofetil

Clinical Transplantation
M NakamuraJ Burdick

Abstract

The regulatory benefit of apoptosis (activation-induced cell death, AICD) in T cells may be impacted by immunosuppressive agents. We examined this for mycophenolate mofetil (MMF) compared with cyclosporine (CYA). Peripheral blood leukocytes (PBL) were stimulated by either Staph enterotoxin B (SEB) or by anti-CD3 plus anti-CD28. Cell division analysis (sequential reduction in carboxyflourescein diacetate succinimidyl ester, CFSE) was used to measure proliferation and determine status of different cell generations. Apoptosis was measured by annexin V staining, and FasL expression by anti-FasL antibody staining, of activated cells using flow cytometry. CSA and mycophenolic acid (MPA, the active agent of MMF) were added in titration in 3-day cultures. We found that CSA caused diminution in apoptosis but MPA increased it with SEB stimulation. The CSA effect on apoptosis was present when a more calcineurin-dependent stimulus. anti-CD3+ anti-CD28, was used but the MPA effect was less, producing a decrease only in the undivided cells. To look more directly at the differential effect on calcineurin-dependent AICD gene induction of the two agents, we measured Fas-L expression with anti-CD-3 + CD28 stimulation, and confirmed that CYA caus...Continue Reading

Citations

Jun 6, 2009·Transplant Infectious Disease : an Official Journal of the Transplantation Society·M L Ritter, L Pirofski
Jun 15, 2007·Expert Opinion on Pharmacotherapy·Eric S White, Joseph P Lynch
Mar 15, 2018·PloS One·Ellen GoMervin C Yoder
Jun 26, 2004·Clinical Transplantation·K TakahashiJ Burdick
Nov 19, 2011·Immunotherapy·Tania C González-Rivera, Joseph McCune
Aug 15, 2001·Transplantation·N OgawaJ F Burdick
Mar 1, 2011·Journal of the American Society of Nephrology : JASN·Gearoid McMahonDidier A Mandelbrot
Jun 18, 2016·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Agnes HacklLutz Thorsten Weber

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis