Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Pedro Negri BernardinoJosé Maurício Sforcin

Abstract

The combination of lower concentrations of antitumor drugs (carboplatin - CARB, doxorubicin - DOX, and methotrexate - MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10-400 μmol/l), DOX (0.5-2 μmol/l) or MET (50-200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting M...Continue Reading

References

Jan 20, 2006·Journal of Veterinary Pharmacology and Therapeutics·B WolfesbergerM Egerbacher
Feb 14, 2006·Clinical & Experimental Metastasis·Ryota TomodaAtsumasa Uchida
Oct 29, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Michelle C BúfaloJosé Maurício Sforcin
Mar 23, 2010·Cancer Chemotherapy and Pharmacology·Luke A WittenburgDouglas H Thamm
Oct 26, 2010·Journal of Ethnopharmacology·José Maurício Sforcin, Vassya Bankova
Oct 13, 2011·The Journal of Pharmacy and Pharmacology·Maria Angélica Ehara WatanabeJosé Maurício Sforcin
Oct 18, 2012·Phytotherapy Research : PTR·N C CinegagliaJ M Sforcin
May 22, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Naiara Costa CinegagliaJosé Maurício Sforcin
May 2, 2015·Journal of Veterinary Internal Medicine·K L Wycislo, T M Fan
Jun 10, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joshua D Schiffman, Matthew Breen
Mar 19, 2016·Phytotherapy Research : PTR·José M Sforcin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis