Positive effects of bFGF modified rat amniotic epithelial cells transplantation on transected rat optic nerve

PloS One
Jia-Xin XieJia-Jun Xu

Abstract

Effective therapy for visual loss caused by optic nerve injury or diseases has not been achieved even though the optic nerve has the regeneration potential after injury. This study was designed to modify amniotic epithelial cells (AECs) with basic fibroblast growth factor (bFGF) gene, preliminarily investigating its effect on transected optic nerve. A human bFGF gene segment was delivered into rat AECs (AECs/hbFGF) by lentiviral vector, and the gene expression was examined by RT-PCR and ELISA. The AECs/hbFGF and untransfected rat AECs were transplanted into the transected site of the rat optic nerve. At 28 days post transplantation, the survival and migration of the transplanted cells was observed by tracking labeled cells; meanwhile retinal ganglion cells (RGCs) were observed and counted by employing biotin dextran amine (BDA) and Nissl staining. Furthermore, the expression of growth associated protein 43 (GAP-43) within the injury site was examined with immunohistochemical staining. The AECs/hbFGF was proven to express bFGF gene and secrete bFGF peptide. Both AECs/hbFGF and AECs could survive and migrate after transplantation. RGCs counting implicated that RGCs numbers of the cell transplantation groups were significantly hig...Continue Reading

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Citations

Dec 26, 2015·International Journal of Molecular Sciences·Yun-Peng WangShao-Chen Xing
Oct 18, 2015·The American Journal of Pathology·Chikako HaradaTakayuki Harada
Sep 14, 2017·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Qi PanFanyong Kong
Dec 7, 2016·Frontiers in Cell and Developmental Biology·Clara Di GermanioBarbara Barboni

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Methods Mentioned

BETA
electrophoresis
ELISA

Software Mentioned

ARRIVE
SAS

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