PMID: 7336145Apr 1, 1981Paper

Positive identification of human leukaemic cells with scanning immuno-electron microscopy, using antibody coated polystyrene (latex) beads as markers

Scandinavian Journal of Haematology
H Gamliel, A Polliack

Abstract

Scanning immuno-electron microscopic labelling techniques were successfully used to identify a variety of leukaemia-lymphoma cells. Anti-T, -B and -common type acute lymphoblastic leukaemia (ALL) antisera were covalently conjugated to polystyrene latex spheres by means of glutaraldehyde. Peripheral blood mononuclear cells obtained from healthy individuals and from patients with T-type ALL, B-type chronic lymphocytic leukaemia (CLL), acute and chronic non-lymphoblastic leukaemias (ANLL) and cultured leukaemia-lymphoma cells were incubated with the above antisera-latex conjugates. Positively labelled cells were easily identified by the presence of latex spheres on their surfaces. Cross reactivity of the antisera used and non-specific attachment of the markers were infrequently encountered. The results of this study confirm earlier scanning electron microscopic reports indicating that different leukaemic cells have characteristic surface features and show that scanning immuno-electron microscopy (SIEM) can readily be applied to the study of human leukaemic cells.

References

Feb 1, 1975·The Journal of Experimental Medicine·R J LejoncF Reyes
Jan 1, 1977·Haematology and Blood Transfusion·H RodtS Thierfelder
Mar 1, 1977·Acta Haematologica·K FischerG Brittinger
May 1, 1975·Clinical Immunology and Immunopathology·M F GreavesT A Lister
Jul 1, 1974·The Journal of Experimental Medicine·A PolliackE De Harven
May 15, 1980·International Journal of Cancer. Journal International Du Cancer·H Ben-BassatA Polliack

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