Abstract
Immunomagnetic CD34(+) cell selection (ICS) is utilized in autologous and allogeneic transplants. In the first case it is used to reduce the neoplastic contamination of concentrates, while in the second case it is needed to carry out a T-depletion of cell concentrates in order to reduce the incidence of graft-versus-host disease (GvHD) in patients who have undergone haplo-identical transplants. The efficacy of CliniMACS technology, after reduction of platelet contamination, incubation of monoclonal antibodies (MAb) and successive washings of concentrates, performed in 16 ICS using the standard method without reducing platelet content, was compared with the use of the automated system CytoMate, which was carried out in 46 ICS. In the group of ICS carried out after automatic manipulation, a significant statistical difference in purity was noted (91.39% versus 83.57, P = 0.017) compared with the group of ICS carried out with the standard procedure. The same significant difference was noted in relation to the remaining percentages of CD3(+) and CD19(+) cells (2.31% versus 5.68%, P = 0.012, and 1.58% versus 2.71%, P = 0.014, respectively). Recovery of CD34+ cells overlapped in the two groups (70.49% versus 68.39%, P = 0.774). Immuno...Continue Reading
References
Sep 15, 1998·Journal of Hematotherapy·C J StainerN H Russell
Mar 2, 1999·British Journal of Haematology·M T VosoR Haas
Apr 16, 2002·Cytotherapy·P V O'DonnellS J Noga
Jul 9, 2002·British Journal of Haematology·Michael J WattsDavid C Linch
Jul 18, 2002·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Antonio BrunoSergio Amadori
Mar 25, 2004·Blood·Pietro SodaniGuido Lucarelli
Dec 15, 2004·Journal of Clinical Apheresis·Fikret ArpaciBekir Oztürk
Jan 26, 2006·Stem Cells and Development·Claudia Del FanteLaura Salvaneschi
Jan 26, 2006·Stem Cells and Development·Paolo PerseghinAndrea Biondi
Sep 21, 2006·British Journal of Haematology·Bimalangshu R Dey, Thomas R Spitzer
Jan 6, 2007·Stem Cells·Mathias Witzens-HarigAnthony D Ho
May 22, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P ComoliF Locatelli
Oct 24, 2007·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Liang-Piu KohNelson J Chao
Oct 26, 2007·Transfusion·Josefina H DykesStefan Scheding
Nov 28, 2007·Blood Reviews·Guido Lucarelli, Javid Gaziev
Dec 11, 2007·Transfusion·Francesco ZinnoGiancarlo Isacchi
Jan 19, 2008·Cytotherapy·E BraakmanI Slaper-Cortenbach
Sep 25, 2008·Bone Marrow Transplantation·J GazievG Lucarelli
Sep 9, 2008·Bone Marrow Transplantation·X-H LuoX-J Huang
Jan 2, 2009·Journal of Hematology & Oncology·Xiao-jun Huang
Citations
Aug 23, 2011·PloS One·Josefina DykesStefan Scheding
Dec 1, 2012·Stem Cells Translational Medicine·Chy-Anh TranDavid Digiusto
Oct 28, 2015·Transfusion·David F StroncekMarianna Sabatino
Feb 26, 2013·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Maria Cristina ScerpaFrancesco Zinno
Apr 18, 2012·Cytotherapy·Maria Cristina ScerpaFrancesco Zinno
Aug 31, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Carolyn A Keever-TaylorRichard J O'Reilly