PMID: 7024414Jan 1, 1981Paper

Positive immunoselection using antibody-enzyme complexes

Journal of Immunological Methods
E Lakow, R S Basch

Abstract

We have developed an immunoselection technique using catalase-anti-catalase complexes coupled to specific antibodies to protect antigen positive target cells from the lethal effects of H2O2. The antibody-enzyme complexes are bound to the target cells through an antibody bridge with specificity for both the complexes and an anti-target cell antibody. In a model system, Thy-1 positive (RL male 1-3) cells were protected by incubation with rabbit anti-brain-associated theta antigen (BAT), sheep anti-rabbit IgG [F(ab)'2] and catalase-anti-catalase complexes. The amount and composition of the complexes adhering to the cells were measured by dual radiolabeling of the catalase and anti-catalase immunoglobulin. This technique provides a means of identifying and isolating large numbers of cells bearing any antigen for which specific antisera are available.

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Citations

Feb 11, 1983·Journal of Immunological Methods·R S BaschE Lakow
Jan 1, 1984·Pathology·R K Kumar, A W Lykke
Oct 1, 1987·Current Eye Research·L AtallaN A Rao

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