Positive PCNA and Ki-67 Expression in the Testis Correlates with Spermatogenesis Dysfunction in Fluoride-Treated Rats

Biological Trace Element Research
Wen-Peng ZhaoBian-Hua Zhou

Abstract

The present study aimed to evaluate the effect of fluoride (F) on spermatogenesis in male rats. F- at 50 and 100 mg/L was administered for 70 days, after which the testicular and epididymis tissues were collected to observe the histopathological structure under a light microscope. The ultrastructure of the testis and sperm was also examined via transmission electron microscopy. The apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki-67, in the testicular and epididymis tissues, were assayed through immunohistochemistry. F- at 50 and 100 mg/L significantly damaged the structure of the testis and epididymis, and the testis and sperm ultrastructure exhibited various changes, including mitochondrial swelling and vacuolization, and apsilated and raised sperm membrane. F treatment significantly increased spermatogenic cell apoptosis in the testis. PCNA (P < 0.01) and Ki-67 (P < 0.01) also presented positive expression in the testis. By comparison, no significant changes occurred in the epididymis. In summary, excessive F intake results in spermatogenesis dysfunction...Continue Reading

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Citations

Sep 15, 2018·Biological Trace Element Research·Hong-Wei WangBian-Hua Zhou
Jun 20, 2018·Biological Trace Element Research·Zhiyuan FengJianhai Zhang
Aug 4, 2019·Environmental Science and Pollution Research International·Ahmed Hamed ArishaMohamed M A Hussein
Nov 17, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hua YangYanli Zhang
Mar 14, 2021·Tissue Engineering and Regenerative Medicine·Heba F IbrahimAmira Y Medwar
Sep 22, 2020·Reproductive Toxicology·Romina PagottoMariela Bollati-Fogolín
Aug 10, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Derya KarabulutBirkan Yakan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis