Positron emission tomography imaging of endometrial cancer using engineered anti-EMP2 antibody fragments.

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
M FuMadhuri Wadehra

Abstract

As imaging of the cell surface tetraspan protein epithelial membrane protein-2 (EMP2) expression in malignant tumors may provide important prognostic and predictive diagnostic information, the goal of this study is to determine if antibody fragments to EMP2 may be useful for imaging EMP2 positive tumors. The normal tissue distribution of EMP2 protein expression was evaluated by immunohistochemistry and found to be discretely expressed in both mouse and human tissues. To detect EMP2 in tumors, a recombinant human anti-EMP2 minibody (scFv-hinge-C(H)3 dimer; 80 kDa) was designed to recognize a common epitope in mice and humans and characterized. In human tumor cell lines, the antibody binding induced EMP2 internalization and degradation, prompting the need for a residualizing imaging strategy. Following conjugation to DOTA (1,4,7,10-tetraazacyclododecane-N,N',N',N'″-tetraacetic acid), the minibody was radiolabeled with (64)Cu (t (1/2) = 12.7 h) and evaluated in mice as a positron emission tomography (PET) imaging agent for human EMP2-expressing endometrial tumor xenografts. The residualizing agent, (64)Cu-DOTA anti-EMP2 minibody, achieved high uptake in endometrial cancer xenografts overexpressing EMP2 (10.2 ± 2....Continue Reading

References

Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H SunadaC L MacLeod
Apr 1, 1997·IEEE Transactions on Medical Imaging·M DefriseD F Newport
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·A M WuS S Gambhir
Jan 31, 2002·Annual Review of Medicine·Johannes Czernin, Michael E Phelps
Sep 5, 2002·Nature Reviews. Cancer·Sanjiv Sam Gambhir
Apr 25, 2003·Experimental and Molecular Pathology·Madhuri WadehraLee Goodglick
Dec 3, 2003·Molecular Imaging·Andreas Markus Loening, Sanjiv Sam Gambhir
Mar 5, 2004·Journal of Medicinal Chemistry·C Andrew BoswellCarolyn J Anderson
Jun 19, 2004·American Journal of Respiratory Cell and Molecular Biology·Katherine DahlinLeland Dobbs
Jul 21, 2004·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Alexander StahlMarkus Schwaiger
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeffrey Y C WongAndrew A Raubitschek
Sep 10, 2004·Abdominal Imaging·A StahlW A Weber
Oct 20, 2004·Seminars in Nuclear Medicine·Heiko Schöder, Steven M Larson
Feb 3, 2005·Nature Reviews. Immunology·Shoshana Levy, Tsipi Shoham
Feb 21, 2006·Developmental Biology·Madhuri WadehraCarmen J Williams
Jan 16, 2007·Current Opinion in Obstetrics & Gynecology·Chyong-Huey LaiTing-Chang Chang
Jul 5, 2007·The Journal of Biological Chemistry·Ashley ForbesJonathan Braun
Jul 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Adam F PrasanphanichCharles J Smith
Apr 11, 2008·Reproductive Biology and Endocrinology : RB&E·Madhuri WadehraJonathan Braun
May 13, 2008·Investigative Ophthalmology & Visual Science·Shawn A MoralesLynn K Gordon
Nov 18, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kaori ShimazakiMadhuri Wadehra
Jan 8, 2009·Bioconjugate Chemistry·Tapan K Nayak, Martin W Brechbiel
Mar 18, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ursula Elsässer-BeileBernd J Pichler
May 15, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Gang NiuXiaoyuan Chen
Jun 6, 2009·Investigative Ophthalmology & Visual Science·Shawn A MoralesLynn K Gordon
Jun 18, 2009·Accounts of Chemical Research·Monica Shokeen, Carolyn J Anderson
Aug 20, 2009·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Tove OlafsenAnna M Wu
Jun 29, 2010·Cancer Biotherapy & Radiopharmaceuticals·Katelyn E McCabe, Anna M Wu
Jul 16, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Bhaswati BaratAnna M Wu
Jul 31, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maoyong FuMadhuri Wadehra

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Datasets Mentioned

BETA
AAC51779.1
AAK29076.1

Methods Mentioned

BETA
phage display
xenograft
xenografts
transfection
PCR
enzyme-linked immunosorbent assay
ELISA
flow cytometry
surgical resection
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad
AMIDE

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