Possibilities of gutta-percha-centered infection in endodontically treated teeth: an in vitro study

Journal of Endodontics
Saji GeorgeAnil Kishen

Abstract

Implanted biomaterials could provide surface for bacterial adherence and formation of biofilm, eventually leading to biomaterial-centered infections (BCIs). In this in vitro study, we examined the biofilm-forming capacity of Enterococcus faecalis on gutta-percha (GP) points under different nutrient status and surface conditioning with saliva and serum. GP points (n = 420) coated with different root canal sealers (Sealapex or Roth) were conditioned with saliva or serum for 2-, 4-, and 12-week intervals and subsequently were incubated with E. faecalis for 2 weeks under nutrient-rich and nutrient-deprived environments. The biofilm-forming capacity and the nature of biofilm formed on GP were assessed by using a viable cell assay and scanning electron microscopy (SEM). E. faecalis produced biofilms on GP points (with and without root canal sealers) under both nutrient-rich and nutrient-deprived environments after conditioning with saliva or serum. SEM reflected that the biofilms formed under nutrient-rich conditions were regular, whereas the biofilms formed under nutrient-deprived conditions were irregular and scanty. Under nutrient-deprived conditions, longer conditioning periods in saliva or serum were required for bacterial adher...Continue Reading

Citations

Feb 14, 2013·Journal of Endodontics·Juliane Maria Guerreiro-TanomaruMário Tanomaru-Filho
Aug 19, 2011·Journal of Endodontics·Christian SengesAli Al-Ahmad
Jun 11, 2016·Molecular Oral Microbiology·M ZehnderG N Belibasakis

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