PMID: 7520786Apr 1, 1994Paper

Possible correlation of b3-a2-type bcr-abl messenger RNA defined by semiquantitative RT-PCR to platelet and megakaryocyte counts in Philadelphia-positive chronic myelogenous leukemia

Internal Medicine
K InokuchiT Nomura

Abstract

Thirty-five patients with Philadelphia chromosome (Ph1)-positive chronic myelogenous leukemia (CML) were classified on the basis of the fusion pattern of bcr-abl mRNA determined by the reverse-transcriptase-polymerase chain reaction (RT-PCR) method. Semiquantitative assay of the bcr exon 2/abl exon 2 fused mRNA (b2-a2) and bcr exon 3/abl exon 2 fused mRNA (b3-a2) resulted in 21 patients showing b3-a2 type mRNA, seven showing b2-a2 type and seven showing coexpression. Quantification of the autoradiographic signals of amplified products was estimated using an MCID image analysis system. The relative intensity was defined as the ratio of bcr-abl signal to that of beta-actin. The relationship between the semiquantified bcr-abl mRNA and the platelet/megakaryocyte counts was analyzed. A possible correlation was found between the semiquantified b3-a2 type mRNA and the platelet (p < .05, N = 28) and megakaryocyte (p < .05, N = 13) counts of these patients. This finding suggests the possibility that b3-a2 mRNA may affect the thrombopoietic activity in Ph1-positive CML in a dose-response manner.

Citations

Feb 15, 2002·International Journal of Hematology·Hiroki YamaguchiKazuo Dan
Apr 8, 1998·Cancer Genetics and Cytogenetics·H YamaguchiK Dan
Feb 5, 1998·British Journal of Haematology·K TajikaS Asano
Jul 16, 2009·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Naoya UchidaTakashi Shimada
Dec 5, 2009·Best Practice & Research. Clinical Haematology·Jane Apperley
Dec 1, 2017·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Albert N HuhoJyotinder N Punia

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