Possible dysregulation of chaperon and metabolic proteins in cystic fibrosis bronchial tissue

Proteomics
Thomas FrischerG Lubec

Abstract

Cystic fibrosis (CF) is an autosomal recessive disease due to mutations of the CF transmembrane conductance regulator gene. A systematic approach to generate a protein expressional pattern in CF bronchial tissue has not been performed so far. It was the aim of this hypothesis-generating study to construct differential proteomes of bronchial biopsies in controls (n = 8) and CF patients (n = 9). Biopsies (pools of three per patient) were taken; proteins were extracted and run on 2-DE with subsequent in-gel digestion and mass spectrometrical identification and quantification of proteins using specific software. Three hundred sixty-six protein spots were identified and compared between groups. Following an approach for multiple testing correction, the chaperone 75 kDa glucose-regulated protein and ubiquinol-cytochrome c reductase complex core protein I and one form of nidogen, a pseudogene of aconitase 2, were increased in CF (p < 0.005). Aberrant protein levels may reflect molecular changes of CF as well as CF-linked inflammation, infection and cellular stress response.

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Citations

Jan 5, 2007·Proceedings of the American Thoracic Society·Yurong GuoJennifer E Van Eyk
Jul 27, 2010·Expert Review of Proteomics·Mark J HendersonPamela L Zeitlin
Mar 5, 2016·Clinical Proteomics·Prue M Pereira-Fantini, David G Tingay
Jul 18, 2009·Neuropharmacology·Lin LiGert Lubec
Jan 31, 2008·Biochimica Et Biophysica Acta·Wei-Qiang ChenGert Lubec
Nov 27, 2007·Neurochemistry International·Wei-Fei DiaoGert Lubec
Jun 16, 2007·Biochimica Et Biophysica Acta·Wei-Fei DiaoGert Lubec
Apr 12, 2007·Hippocampus·Wei-Fei DiaoGert Lubec
Nov 1, 2007·Proteomics. Clinical Applications·Wei-Fei DiaoGert Lubec
Aug 15, 2009·Journal of Cellular Physiology·Andy T Y Lau, Jen-Fu Chiu
Jan 10, 2012·Biochimica Et Biophysica Acta·Francesco GalliUNKNOWN Working Group on Inflammation in Cystic Fibrosis
Aug 6, 2020·International Journal of Molecular Sciences·Nara LiessiClarissa Braccia
Feb 10, 2010·Journal of Proteome Research·Leila Afjehi-SadatGert Lubec
Jan 22, 2009·Journal of Proteome Research·Jae-Kyung MyungGert Lubec

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