Possible Escape of a Recombinant Isolate of Potato virus Y by Serological Indexing and Methods of its Detection

Plant Disease
Rudra SinghMathuresh Singh

Abstract

Surveys of commercial and seed potato fields for virus diseases (1998 to 2002) in Manitoba established that Potato virus Y (PVY) is of concern in seed potato production. To determine the prevalence of PVY strains, PVY-infected tubers identified by reverse transcription-polymerase chain reaction (RT-PCR) from surveys (2000 to 2001) were grown for symptom expression and strain characterization by strain-specific RT-PCR, bioassays, and serological assays. Of the samples collected (2000 to 2001) and tested by RT-PCR, 4.0% contained PVY. Further analysis of the PVY-positive samples by a duplex RT-PCR facilitating the simultaneous detection of common (PVYO) and tobacco veinal necrosis strains (PVYN/NTN) indicated that 37.5% contained PVYO and 63.5% contained PVYN-type isolates. Analysis of the PVYN-type samples using three monoclonal antibodies (MAbs) showed that all reacted with only the PVYO MAbs and not with the PVYN-specific MAb. Partial nucleotide sequences of both ends of PVY-RNA showed that the PVYN-type isolates resembled those reported in 1996 from Manitoba. These isolates are designated as PVYN:O. In view of the increased incidence of PVYN:O in one production area, seed tubers imported from other provinces of Canada and the...Continue Reading

References

Apr 1, 1995·The Journal of General Virology·V M TordoS Astier-Manifacier

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Citations

Jul 21, 2012·Journal of Economic Entomology·Rajagopalbabu SrinivasanJonathan L Whitworth
Aug 21, 2009·The Journal of General Virology·Xiaojun HuJim H Lorenzen

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