Possible indirect detection of rHuEPO administration in human urine by high-performance liquid chromatography tandem mass spectrometry

European Journal of Mass Spectrometry
Svetlana A AppolonovaGrigory M Rodchenkov

Abstract

The present study is based on the assumption that changes in an ADMA-DDAH-NOS (ADMA-asymmetrical dimethylarginine; DDAH-dimethyl-arginine dimethylaminohydrolase; NOS-nitric oxide synthase) system could be employed as indirect markers for recombinant human erythropoietin (rHuEPO) administration in doping control. We assessed a predictive value of four proposed new markers for rHuEPO abuse. Preliminary data showed that concentrations of ADMA, symmetrical dimethylarginine (SDMA), citrulline and arginine in human urine were increased after administration of a single intravenous erythropoietin injection (2000 U day(-1), Epocrine, St-Petersburg, Russia). The study of variations of ADMA, SDMA, arginine and citrulline levels before and after rHuEPO administration was performed with two healthy male volunteers. Urine samples were collected before rHuEPO administration and urinary concentrations of ADMA and SDMA were determined at 10.0-40 microg mL(-1) and of arginine and citrulline at 0.5-10 microg mL(-1). A single dose injection of rHuEPO caused an increase in ADMA, SDMA, arginine and citrulline concentrations up to 40-270 microg mL(-1), 40-240 microg mL(-1), 10-60 microg mL(-1) and 12-140 microg mL(-1), respectively. These preliminary...Continue Reading

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Citations

Aug 25, 2011·Forensic Science International : Synergy·Christian Reichel
Nov 12, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jens Martens-Lobenhoffer, Stefanie M Bode-Böger
Nov 26, 2010·Therapeutic Drug Monitoring·Christina TsitsimpikouAristides Tsatsakis
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ksenia M ShestakovaSvetlana A Appolonova
Dec 22, 2020·Frontiers in Sports and Active Living·Raphael Faiss, David Pavot

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