Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyrene/Ethanol CoExposure

Oxidative Medicine and Cellular Longevity
Simon BucherB Fromenty

Abstract

Exposure to xenobiotics could favor the transition of nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis in obese patients. Recently, we showed in different models of NAFL that benzo[a]pyrene (B[a]P) and ethanol coexposure induced a steatohepatitis-like state. One model was HepaRG cells incubated with stearate and oleate for 2 weeks. In the present study, we wished to determine in this model whether mitochondrial dysfunction and reactive oxygen species (ROS) overproduction could be involved in the occurrence of this steatohepatitis-like state. CRISPR/Cas9-modified cells were also used to specify the role of aryl hydrocarbon receptor (AhR), which is potently activated by B[a]P. Thus, nonsteatotic and steatotic HepaRG cells were treated with B[a]P, ethanol, or both molecules for 2 weeks. B[a]P/ethanol coexposure reduced mitochondrial respiratory chain activity, mitochondrial respiration, and mitochondrial DNA levels and induced ROS overproduction in steatotic HepaRG cells. These deleterious effects were less marked or absent in steatotic cells treated with B[a]P alone or ethanol alone and in nonsteatotic cells treated with B[a]P/ethanol. Our study also disclosed that B[a]P/ethanol-induced impairment of mitochondrial ...Continue Reading

References

Mar 1, 1985·The Journal of Cell Biology·P GreenspanS D Fowler
Jan 1, 1995·Pharmacology & Therapeutics·B Fromenty, D Pessayre
Oct 7, 1998·Chemical Research in Toxicology·T ShimadaF P Guengerich
Oct 9, 1999·Free Radical Biology & Medicine·S M BaileyC C Cunningham
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y ShimizuT Ishikawa
Apr 21, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N KazerouniN Rothman
Sep 19, 2002·The Journal of Biological Chemistry·Julie St-PierreMartin D Brand
Jul 29, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maurice G EmeryKenneth E Thummel
Apr 30, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mary Lynn BajtHartmut Jaeschke
Dec 9, 2004·The Journal of Veterinary Medical Science·Zein ShabanShoichi Fujita
May 12, 2005·Journal of Hepatology·Dominique Pessayre, Bernard Fromenty
Mar 21, 2006·Archives of Biochemistry and Biophysics·E C HenryT A Gasiewicz
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Inmaculada García-RuizJosé A Solís-Herruzo
Jul 27, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Inmaculada García-RuizJosé A Solís-Herruzo
Dec 15, 2007·Free Radical Biology & Medicine·Yongke Lu, Arthur I Cederbaum
Oct 8, 2009·The American Journal of Pathology·Claudia MitchellHélène Gilgenkrantz
Sep 14, 2010·Archives of Biochemistry and Biophysics·Yuki KawanoMasaru Yoshida
Oct 12, 2010·PPAR Research·Maryam RakhshandehrooSander Kersten
Dec 15, 2010·Journal of Hepatology·Tarek I Abu-Rajab TamimiAriel E Feldstein
Feb 15, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mitchell R McGillHartmut Jaeschke
Mar 11, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sébastien AnthérieuMarie-Anne Robin
Jun 15, 2011·Clinics and Research in Hepatology and Gastroenterology·J AubertB Fromenty
Aug 19, 2011·Environmental Health Perspectives·Chun WangShelley A Tischkau
Jan 10, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karima BegricheBernard Fromenty
Oct 2, 2013·Ageing Research Reviews·Christiaan F Labuschagne, Arjan B Brenkman
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Nov 26, 2013·Journal of Hepatology·Lena Maria PawellaBeate Katharina Straub
Jan 5, 2014·Environmental Science and Pollution Research International·Ola WestmanMagnus Engwall
Mar 1, 2014·Liver International : Official Journal of the International Association for the Study of the Liver·Anaïs MichautBernard Fromenty
Mar 7, 2014·Journal of Lipid Research·Xu XuAnn-Hwee Lee
Mar 8, 2014·World Journal of Gastroenterology : WJG·Fatiha Nassir, Jamal A Ibdah
May 28, 2014·Methods in Enzymology·Aleksandra WojtalaMariusz R Wieckowski
Sep 10, 2014·Best Practice & Research. Clinical Gastroenterology·Peter Dietrich, Claus Hellerbrand
Oct 24, 2014·World Journal of Gastroenterology : WJG·Giuseppe ParadiesGiuseppe Petrosillo
Nov 6, 2014·Oxidative Medicine and Cellular Longevity·Aaron M GusdonShen Qu
Jul 15, 2015·Chemical Research in Toxicology·An T VuClifford H Watson
Nov 17, 2015·The Journal of Clinical Investigation·Santhosh SatapatiShawn C Burgess

❮ Previous
Next ❯

Citations

May 28, 2019·Current Environmental Health Reports·Banrida WahlangMatthew C Cave
Apr 27, 2019·Scientific Reports·Mohammad A RahmanSantosh Kumar
May 16, 2019·Oxidative Medicine and Cellular Longevity·Tong LiYong Zhang
May 20, 2020·International Journal of Molecular Sciences·Juliette LeglerJorke H Kamstra
Oct 7, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Arnaud TêteDominique Lagadic-Gossmann
Nov 3, 2021·Journal of Cachexia, Sarcopenia and Muscle·Trace ThomeRussell T Hepple

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
electrophoresis

Software Mentioned

GraphPad Prism
GraphPad
ImageXpress

Related Concepts

Related Feeds

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.