Possible involvement of reactive oxygen species in D-galactosamine-induced sensitization against tumor necrosis factor-alpha-induced hepatocyte apoptosis

Journal of Cellular Physiology
Y OsawaS Nakashima

Abstract

Intravenous administration of tumor necrosis factor-alpha (TNF-alpha) (0.5 microg/mouse) caused hepatocyte apoptosis in BALB/c mice when they were sensitized with D-galactosamine (GalN, 20 mg/mouse). Activation of nuclear factor kappa B (NF-kappa B) and expression of apoptotic Bcl-2 family members were not significantly different between livers of mice treated with TNF-alpha alone and GalN + TNF-alpha, indicating that neither activation of NF-kappa B nor expression of Bcl-2 family is involved in the sensitization by GalN against TNF-alpha-induced hepatocyte apoptosis. To identify differentially expressed genes implicated in GalN-induced hepatocyte sensitization, we adopted mRNA fingerprinting using an arbitrarily primed polymerase chain reaction. The present analysis revealed that mRNA expression of extracellular antioxidant, selenoprotein P, was up-regulated in the livers after GalN administration. GalN-induced increase in its protein level was confirmed by Western blotting. Increased expression of this gene was also observed in the liver of mice treated with concanavalin A, but not anti-Fas antibody. mRNA of another antioxidant, glutathione peroxidase-1, was also up-regulated, and lipid peroxides were produced in the liver af...Continue Reading

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Citations

Dec 10, 2002·Antioxidants & Redox Signaling·Mark J Czaja
Aug 29, 2012·Antioxidants & Redox Signaling·Yoshiyuki MatsuoJunji Yodoi
Aug 25, 2007·Journal of Gastroenterology and Hepatology·Rajat Singh, Mark J Czaja
Oct 13, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Yosuke OsawaDavid A Brenner
Dec 3, 2014·Redox Biology·Jörn M Schattenberg, Mark J Czaja
Jun 16, 2004·FEMS Immunology and Medical Microbiology·Akiko MorikawaTakashi Yokochi
May 18, 2010·Journal of Pain and Symptom Management·Jennifer L SteelT Clark Gamblin
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Sep 7, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Aránzazu SánchezSnorri S Thorgeirsson
Sep 9, 2006·Journal of Cellular Biochemistry·Fukka YouShigeru Nakashima
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Dec 1, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yosuke OsawaDavid A Brenner
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Apr 19, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yosuke OsawaShigeru Nakashima
Mar 23, 2010·European Journal of Pharmacology·Seok-Joo KimSun-Mee Lee
Aug 25, 2004·Biochemical and Biophysical Research Communications·John J Haddad

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