PMID: 2506164Jul 1, 1989Paper

Possible mechanisms for the revival of glutaraldehyde-treated spores of Bacillus subtilis NCTC 8236

The Journal of Applied Bacteriology
E G PowerA D Russell

Abstract

Spores of Bacillus subtilis NCTC 8236 were exposed to 2% alkaline glutaraldehyde and subsequently subjected to various treatments in an attempt to revive injured spores. Treatment with alkali (sodium or potassium hydroxide or, to a lesser extent, sodium bicarbonate) proved to be most successful. Some revival was achieved after thermal treatment. No revival was obtained with lysozyme or with various types of coat-removing agents. Experiments designed to distinguish between germination and outgrowth in the revival process established that sodium hydroxide (optimum concentration, 20 mmol/l) added to glutaraldehyde-treated spores increased the potential for germination. In contrast, spores which had been allowed to germinate before exposure to low concentrations of glutaraldehyde and then to sodium hydroxide were inhibited at the outgrowth phase to a much greater extent than germinated spores treated with the dialdehyde without subsequent alkali exposure. The results overall are discussed in terms of the possible mechanism and site of action of glutaraldehyde and the practical implications and significance of its use as a sporicide.

References

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Citations

Dec 19, 2000·Journal of Applied Microbiology·N A TurnerD Lloyd
Jul 1, 1993·The Journal of Applied Bacteriology·N D Williams, A D Russell
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·S F Bloomfield, M Arthur
Sep 1, 1989·The Journal of Applied Bacteriology·E G Power, A D Russell
Feb 19, 2002·Letters in Applied Microbiology·T HamoudaJ R Baker
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Nov 1, 1992·Applied and Environmental Microbiology·M ChambonH Peigue-Lafeuille
Apr 1, 1990·Clinical Microbiology Reviews·A D Russell

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