Possible Mitochondria-Associated Enzymatic Role in Non-Hodgkin Lymphoma Residual Disease.

Cancer Growth and Metastasis
Ian KusaoBruce Shiramizu

Abstract

BACKGROUND: The mechanisms responsible for resistant or recurrent disease in childhood non-Hodgkin lymphoma (NHL) are not yet fully understood. A unique mechanism suggesting the role of the mitochondria as the key energy source responsible for residual cells has been assessed in the clinical setting on specimens from patients on therapy were found to have increased copies of mitochondrial DNA (mtDNA) associated with positive minimal residual disease and/or persistent disease (MRD/PD) status. The potential role of mtDNA in MRD/PD emphasizes queries into the contributions of relevant enzymatic pathways responsible for MRD/PD. This study hypothesized that in an in-vitro model, recovering or residual cells from chemotoxicity will exhibit an increase in both citrate synthase and isocitrate dehydrogenase expression and decrease in succinate dehydrogenase expression. PROCEDURE: Ramos cells (Burkitt lymphoma cell line) were exposed to varying concentrations of doxorubicin and vincristine for 1 hr; and allowing for recovery in culture over a 7-day period. cDNA was extracted on days 1 and 7 of the cell culture period to assess the relative expression of the aforementioned genes. RESULTS: Increase citrate synthase, increase isocitrate d...Continue Reading

References

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Citations

Jan 4, 2012·Fundamental & Clinical Pharmacology·Kham R M MarthaSurya B Prasad
Sep 14, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Surya B Prasad, Akalesh K Verma
Jul 2, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Birte ArltHedwig E Deubzer

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BETA
PCR

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StepOne Plus
Primer Express

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