Possible modulating actions of plant extracts on the chromosome breaking activity of MMC and Ara-C in human lymphocytes in vitro

Toxicology in Vitro : an International Journal Published in Association with BIBRA
T RoncadaM S Mantovani

Abstract

Plants popularly used as medicine have been seen as promising natural agents by the pharmaceutical industry. In the present study the action of Psidium guajava L. (Pg) and Achillea millefolium L. (Am) infusions on chromosomal aberration formation in human lymphocyte system in vitro was assessed, associating them with the alkylating agent mitomycin C (MMC) and the DNA repair inhibitor cytosine-beta-arabin-furanoside (Ara-C). The cells were cultivated for 72 h and treated continuously with Pg and the Am infusions at dosages of 2.62 x 10(-4) g and 3.5 x 10(-4) g/ml culture medium, respectively. Treatments with MMC (0.30 microg/ml) or Ara-C (5 x 10(-7) microg/ml) were administered after 48 h of cell culture. Each samples (five individual) were exposed to nine treatments (control with PBS; Pg; Am; MMC; MMC+Pg; MMC+Am; Ara-C; Ara-C+Pg; and Ara-C+Am) and 100 cells were analyzed per cell culture. The used doses of each infusion did not cause clastogenic effects significantly different to the negative control (control=1%; Pg=2.2%; Am=1.8%). Nevertheless, the aberrant cell frequency after MMC treatment was significantly increased by the Am infusion (MMC=32.4%; MMC+Pg=36.2%; MMC+Am=44%), especially when the chromatid break types number wa...Continue Reading

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Citations

Feb 9, 2012·Bulletin of Environmental Contamination and Toxicology·Süleyman GülPinar Aksu
Jul 22, 2011·Drug and Chemical Toxicology·Mehmet BuyukleylaCengiz Darici
Nov 15, 2013·Drug and Chemical Toxicology·Nadire Sevindik, Eyyup Rencuzogullari
Jan 19, 2016·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Luis Francisco RossiMarta Dolores Mudry
Aug 28, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yasir Hasan SiddiqueMohammad Afzal
Jun 18, 2014·Chemistry & Biodiversity·Miroslav NovakovićSlobodan Milosavljević
Dec 3, 2005·Biosensors & Bioelectronics·Amelita BartolomeJames Villanueva
Feb 6, 2017·Nutrients·Jeannett Alejandra Izquierdo-VegaEduardo Madrigal-Santillán

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