Possible nystatin-protein interaction in yeast plasma membrane vesicles in the presence of ergosterol. A Förster energy transfer study

FEBS Letters
M OpekarováE Amler

Abstract

The Förster energy transfer from tryptophan residues of membrane proteins to nystatin was measured in reconstituted yeast plasma membrane vesicles free of, or doped with, ergosterol. We wanted to elucidate whether the functional change of membrane transport proteins from H+ symporters to facilitators, observed in ergosterol-containing plasma membrane vesicles on addition of nystatin [Opekarová and Tanner (1994) FEBS Lett. 350, 46-50], is reflected in altered protein-nystatin relations within the membrane. Both frequency-domain and time-domain time-resolved fluorescence spectroscopy showed that in the presence of ergosterol nystatin is located much closer to membrane proteins than in its absence.

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Related Concepts

Plasma Membrane
Energy Transfer
Lumisterol
Statistical Programs, Computer Based
Cell Surface Proteins
Stamycin
Plasma Protein Binding Capacity
Saccharomyces cerevisiae
PMS-Tryptophan

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