Jul 1, 1989

Possible origin of glutamatergic projections to the midbrain periaqueductal gray and deep layer of the superior colliculus of the rat

Brain Research Bulletin
A J Beitz

Abstract

The possible origin of glutamatergic input to the rodent periaqueductal gray (PAG) was analyzed utilizing a combined retrograde transport-immunocytochemical technique. Injections of wheat germ agglutinin-horseradish peroxidase were made into the PAG of 12 adult rats and into the deep layer of the superior colliculus in 2 rats. The brain tissue was first reacted histochemically to demonstrate the retrograde tracer and subsequently processed with immunohistochemical techniques using a recently developed monoclonal glutamate antibody. Following PAG injections, several brain areas were found to contain double-labeled neurons. The greatest number of double-labeled glutamate-like immunoreactive neurons were observed in the zona incerta, spinal trigeminal nucleus, cuneiform nucleus, cingulate cortex, cerebellar interpositus nucleus, deep mesencephalic nucleus and the PAG itself. Double-labeled neurons were also observed in several other nuclei including the pretectal nuclei, the frontal and occipital cortex, several reticular nuclei, the dorsomedial hypothalamic nucleus, and the substantia nigra. Many of the same nuclei contained double-labeled neurons following collicular injections, but in addition, double-stained cells were found i...Continue Reading

Mentioned in this Paper

Alpha-Peroxidase
Optic Lobe, Human
Occipital Lobe
Pregeniculate Nucleus
Projections and Predictions
Wheat Germ Agglutinin Isolectin 2
August Rats
Neurons
Brain
Central Gray Substance of Midbrain

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